Chi M M, Manchester J K, Yang V C, Curato A D, Strickler R C, Lowry O H
Department of Pharmacology, Washington University Medical School, St. Louis, Missouri 63110.
Biol Reprod. 1988 Sep;39(2):295-307. doi: 10.1095/biolreprod39.2.295.
A methodology is described for analyzing single human ova for 8 or 9 different metabolic enzymes, or 4 or 5 enzymes plus as many metabolites. This overcomes an obstacle to the study of human ovum metabolism: the severe limitation of usable material. Results obtained with this methodology, applied to discarded specimens from an in vitro fertilization program, indicate that in spite of imperfections these ova can provide a valid picture of the metabolic characteristics of normal human ova. Data are presented for 17 enzymes from 8 metabolic pathways in human and mouse ova. Relative to size, 10 of the enzymes were substantially higher in human than mouse ova. Most dramatically so were 2 enzymes of fatty acid metabolism (10-fold and 15-fold), hexokinase (9-fold), and aspartate aminotransferase (19-fold). This suggests that major species differences in metabolism are present. The validity of the human data, in spite of restriction to discarded material, is supported by (1) consistency of results among most of the ova, 2] concordance between average levels with those of rare specimens that were discarded because sperm were not available, and (3) the presence of adenosine triphosphate (ATP) concentrations similar to those of normal mouse ova. Surprisingly, both human and mouse ova contain phosphocreatine at levels nearly equal of those of ATP.
本文描述了一种用于分析单个人类卵子中8种或9种不同代谢酶，或4种或5种酶以及尽可能多代谢物的方法。这克服了人类卵子代谢研究中的一个障碍：可用材料的严重限制。将该方法应用于体外受精项目废弃标本所获得的结果表明，尽管存在缺陷，但这些卵子仍能提供正常人类卵子代谢特征的有效图景。文中给出了人类和小鼠卵子中8条代谢途径的17种酶的数据。相对于大小而言，人类卵子中10种酶的含量显著高于小鼠卵子。脂肪酸代谢的2种酶（分别高10倍和15倍）、己糖激酶（高9倍）和天冬氨酸转氨酶（高19倍）的差异最为显著。这表明存在主要的物种代谢差异。尽管限于废弃材料，但人类数据的有效性得到了以下几点支持：（1）大多数卵子的结果具有一致性；（2）平均水平与因无精子而废弃的罕见标本的水平一致；（3）三磷酸腺苷（ATP）浓度与正常小鼠卵子相似。令人惊讶的是，人类和小鼠卵子中的磷酸肌酸水平几乎与ATP相等。
