Department of Chemistry, Indian Institute of Technology, Kharagpur, India.
Department of Chemistry, Boston College, Chestnut Hill, Massachusetts.
Mass Spectrom Rev. 2019 Jan;38(1):3-21. doi: 10.1002/mas.21545. Epub 2017 Oct 13.
The MALDI-based mass spectrometry, over the last three decades, has become an important analytical tool. It is a gentle ionization technique, usually applicable to detect and characterize analytes with high molecular weights like proteins and other macromolecules. The earlier difficulty of detection of analytes with low molecular weights like small organic molecules and metal ion complexes with this technique arose due to the cluster of peaks in the low molecular weight region generated from the matrix. To detect such molecules and metal ion complexes, a four-prong strategy has been developed. These include use of alternate matrix materials, employment of new surface materials that require no matrix, use of metabolites that directly absorb the laser light, and the laser-absorbing label-assisted LDI-MS (popularly known as LALDI-MS). This review will highlight the developments with all these strategies with a special emphasis on LALDI-MS.
基质辅助激光解吸电离质谱（MALDI-based mass spectrometry）在过去三十年中已成为一种重要的分析工具。它是一种温和的电离技术，通常适用于检测和表征具有高分子量的分析物，如蛋白质和其他生物大分子。由于基质在低分子量区域产生的峰簇，早期用该技术检测低分子量分析物（如小分子有机分子和金属离子配合物）存在困难。为了检测这些分子和金属离子配合物，已经开发了四项策略。这些策略包括使用替代基质材料、使用不需要基质的新型表面材料、使用直接吸收激光光的代谢物，以及激光吸收标签辅助激光解吸电离质谱（通常称为 LALDI-MS）。本文将重点介绍所有这些策略的发展情况，特别强调 LALDI-MS。
