He Ming, Jiang Linlin, Li Bin, Wang Guangbin, Wang Jiashi, Fu Yonghui
Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Department of Electrotheropy, Shenyang Medical College Affiliated Central Hospital, Shenyang, Liaoning, People's Republic of China.
Oncotarget. 2017 May 10;8(39):65100-65110. doi: 10.18632/oncotarget.17783. eCollection 2017 Sep 12.
Studies demonstrated that reduced PTEN levels are associated with poor prognoses of osteosarcoma. The nuclear localization of PTEN is important for its tumor suppressive function. Equally importantly, PTEN is the most significant negative regulator of PI3K/Akt signaling cascade, the constitutively activated pathway in osteosarcoma. In our study MG63 cells and U2OS cells were treated with the indicated concentrations of oxymatrine, in order to find the inhibition of oxymatrine to cells. We found the functions of oxymatrine on proliferation, apoptosis and invasion in cells. Oxymatrine could increase the expression of PTEN and promote its nuclear translocation in MG63 cells. In addition, oxymatrine could induce cell cycle arrest in G1 phase and apoptosis of MG63 cells. The migration and invasion potential of MG63 cells were also markedly inhibited by oxymatrine. Oxymatrine could suppress the growth and invasion of MG63 human osteosarcoma cells by up-regulating PTEN and promoting its nuclear translocation and inhibiting PI3K/Akt signaling pathway.
研究表明，PTEN水平降低与骨肉瘤的不良预后相关。PTEN的核定位对其肿瘤抑制功能很重要。同样重要的是，PTEN是PI3K/Akt信号级联的最重要负调节因子，而PI3K/Akt信号级联是骨肉瘤中持续激活的信号通路。在我们的研究中，用指定浓度的氧化苦参碱处理MG63细胞和U2OS细胞，以研究氧化苦参碱对细胞的抑制作用。我们发现了氧化苦参碱对细胞增殖、凋亡和侵袭的作用。氧化苦参碱可增加MG63细胞中PTEN的表达并促进其核转位。此外，氧化苦参碱可诱导MG63细胞在G1期发生细胞周期阻滞并诱导其凋亡。氧化苦参碱还可显著抑制MG63细胞的迁移和侵袭能力。氧化苦参碱可通过上调PTEN、促进其核转位以及抑制PI3K/Akt信号通路来抑制MG63人骨肉瘤细胞的生长和侵袭。
