A species-specific sperm-activating peptide from the egg jelly of the sea urchin Diadema setosum.

Yoshino K, Kurita M, Yamaguchi M, Nomura K, Takao T, Shimonishi Y, Suzuki N

Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.

Comp Biochem Physiol B. 1990;95(2):423-9. doi: 10.1016/0305-0491(90)90097-d.

Abstract

A species-specific sperm-activating peptide was isolated from the egg jelly of the sea urchin Diadema setosum and the amino acid sequence was determined as follows: (formula; see text). 2. The peptide caused significant increases of respiration rates and cyclic nucleotide concentrations in D. setosum spermatozoa as low as 10(-9) M. 3. The addition of the peptide to D. setosum spermatozoa resulted in the appearance of a newly stained protein (mol. wt 128,000) on sodium dodecyl sulfate-polyacrylamide gels.

摘要