Osman Marwan, El Safadi Dima, Benamrouz-Vanneste Sadia, Cian Amandine, Moriniere Romain, Gantois Nausicaa, Delgado-Viscogliosi Pilar, Guyot Karine, Bosc Stéphanie, Chabé Magali, Petit Thierry, Viscogliosi Eric, Certad Gabriela
Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d'Infection et d'Immunité de Lille, 1 rue du Professeur Calmette, BP 245, 59019, Lille cedex, France.
Laboratoire Microbiologie Santé et Environnement, Ecole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, rue Mitein, Tripoli, Lebanon.
Parasitol Res. 2017 Dec;116(12):3419-3422. doi: 10.1007/s00436-017-5645-1. Epub 2017 Oct 13.
Cryptosporidium represents a major cause of gastrointestinal illness in humans and animals including domestic, wild, and in captivity animals, and more than 30 validated species of Cryptosporidium are recognized as infectious to different hosts such as mammals, birds, reptiles, amphibians, and fish. Therefore, numerous investigations have been conducted worldwide in order to shed light on the epidemiology of this parasite and to explore its potential reservoirs. Few surveys, targeting humans and animals have been carried out regarding the epidemiology of Cryptosporidium spp. in France and no data are available about the circulation of this parasite in French zoological gardens. Herein, we determined the prevalence of Cryptosporidium in animals housed in two French zoos. A total of 307 fecal samples belonging to 161 species were screened by nested PCR. Overall, Cryptosporidium DNA was detected in 1.9% of the 161 species and 1% of the total number of fecal samples tested. Additionally, three Cryptosporidium species were identified: C. galli, C. andersoni, and C. tyzzeri. To our knowledge, this is the first molecular study focused on Cryptosporidium infection in captivity animals in France. This study is of interest considering the exposure of a large number of humans and animals to this waterborne protozoan, found ubiquitously in the environment.
隐孢子虫是人和动物胃肠道疾病的主要病因，这些动物包括家畜、野生动物和圈养动物，已确认有30多种隐孢子虫可感染不同宿主，如哺乳动物、鸟类、爬行动物、两栖动物和鱼类。因此，世界各地开展了大量调查，以阐明这种寄生虫的流行病学情况并探索其潜在宿主。在法国，针对人和动物的隐孢子虫属流行病学调查很少，且尚无关于该寄生虫在法国动物园中传播情况的数据。在此，我们测定了法国两家动物园圈养动物中隐孢子虫的感染率。通过巢式PCR对总共属于161个物种的307份粪便样本进行了筛查。总体而言，在161个物种中的1.9%以及所检测粪便样本总数的1%中检测到了隐孢子虫DNA。此外，鉴定出了三种隐孢子虫：鸡隐孢子虫、安氏隐孢子虫和泰泽隐孢子虫。据我们所知，这是法国首次针对圈养动物隐孢子虫感染开展的分子研究。鉴于大量人和动物接触这种在环境中普遍存在的水源性原生动物，该研究具有重要意义。
