Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, South Korea.
Department of Nephrology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, China.
J Thromb Thrombolysis. 2017 Nov;44(4):556-564. doi: 10.1007/s11239-017-1537-6.
Acute myocardial infarction (AMI) is one of the leading causes of morbidity and mortality worldwide, while early diagnosis still represents an upmost priority. While platelet activation is critical for AMI pathogenesis, the role of platelet microRNAs (pmiRNAs) as biomarkers for AMI is unclear. Furthermore, correlations between the levels of pmiRNAs and indices of platelet activity are also unknown. Expression of platelet miR-1, miR-21, miR-126, miR-150 and miR-223 were prospectively assessed in 20 ST-segment elevation myocardial infarction (STEMI) patients, and 40 healthy volunteers. Platelet reactive units (PRU) were assessed with cartridge analyzer, and vasodilator-stimulated phosphoprotein (VASP) was measured by flow cytometry. There were no significant changes in pmiR-1 expression. Expressions of pmiR-21 and pmiR-126 were decreased, while pmiR-150 and pmiR-223 were increased in STEMI patients when compared to controls (all p < 0.01). However, only pmiR-126 exhibited correlation with plasma cardiac troponin I (r = - 0.556, p = 0.011) in STEMI. There was no correlation between pmiRNAs with PRU or VASP during admission, or at 48 h post-stenting. Among tested pmiRNAs, pmiR-126 may serve as a potential novel biomarker for STEMI, while pmiR-1, pmiR-21, pmiR-150, and pmiR-223 were not particularly useful. Moreover, since assessed pmiRNA expression did not correlate well with platelet activity indices their potential diagnostic utility is quite limited.
急性心肌梗死（AMI）是全球发病率和死亡率的主要原因之一，而早期诊断仍然是当务之急。虽然血小板激活对于 AMI 的发病机制至关重要，但血小板 microRNAs（pmiRNAs）作为 AMI 生物标志物的作用尚不清楚。此外，pmiRNA 水平与血小板活性指数之间的相关性也未知。前瞻性评估了 20 例 ST 段抬高型心肌梗死（STEMI）患者和 40 名健康志愿者的血小板 miR-1、miR-21、miR-126、miR-150 和 miR-223 的表达。用试剂盒分析仪评估血小板反应单位（PRU），并用流式细胞术测量血管扩张刺激磷酸蛋白（VASP）。pmiR-1 的表达没有明显变化。与对照组相比，STEMI 患者的 pmiR-21 和 pmiR-126 表达降低，而 pmiR-150 和 pmiR-223 表达增加（均 p<0.01）。然而，只有 pmiR-126 与 STEMI 中的血浆心肌肌钙蛋白 I 呈负相关（r=-0.556，p=0.011）。在入院期间或支架置入后 48 小时，pmiRNAs 与 PRU 或 VASP 之间没有相关性。在测试的 pmiRNAs 中，pmiR-126 可能是 STEMI 的一种潜在新型生物标志物，而 pmiR-1、pmiR-21、pmiR-150 和 pmiR-223 则没有特别有用。此外，由于评估的 pmiRNA 表达与血小板活性指数相关性不佳，因此其潜在的诊断实用性非常有限。
