Indian Institute of Technology Delhi, Instrument Design Development Centre, Hauz Khas, New Delhi, India.
J Biomed Opt. 2017 Oct;22(10):1-8. doi: 10.1117/1.JBO.22.10.106009.
Red blood cell (RBC) anomalies are significant symptoms for identification of health disorders and several blood diseases, which involve the modification of the parameters and biophysical characteristics of such cells. The aim of this study is to measure the three-dimensional phase information of healthy RBCs and their parameters, such as cell diameter, thickness, and hemoglobin (Hb) content, using Talbot-projected fringes. The Talbot image of linear grating is projected onto an RBC slide. The deformed grating lines due to the shape and refractive index of RBCs are recorded by a CCD camera through a 20× microscope objective. Hilbert transform is used to extract the phase image from the deformed projected grating lines. Experimentally calculated values of diameter (8.2 μm), thickness (2.7 μm), and Hb content (28.7 pg/cell) are well within the limits available in the literature. The proposed system is robust and user-friendly and performs the imaging of RBCs with high axial and lateral resolution (2.19 μm).
红细胞（RBC）异常是识别健康障碍和几种血液疾病的重要症状，其中涉及这些细胞的参数和生物物理特性的改变。本研究旨在使用泰伯投影条纹测量健康 RBC 的三维相位信息及其参数，如细胞直径、厚度和血红蛋白（Hb）含量。线性光栅的泰伯图像被投影到 RBC 载玻片上。通过 20×显微镜物镜，CCD 相机记录由于 RBC 的形状和折射率而产生的变形光栅线。使用希尔伯特变换从变形的投影光栅线中提取相位图像。实验计算得到的直径（8.2μm）、厚度（2.7μm）和 Hb 含量（28.7pg/细胞）的值在文献中可用的范围内。所提出的系统具有鲁棒性和用户友好性，可以实现具有高轴向和横向分辨率（2.19μm）的 RBC 成像。
