Rajkumar Rahul, Bhaya Bhavana, Mamilla Divya, Czech Theresa, Kisseih Esther, Saini Arun, Chouthai Nitin
Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; Bloomberg School of Public Health, Department of International Health, Johns Hopkins University, Baltimore, MD, United States.
Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; University Medical Center, Department of Internal Medicine, University of Nevada-Las Vegas, Las Vegas, NV, United States.
Int J Dev Neurosci. 2018 Apr;65:61-65. doi: 10.1016/j.ijdevneu.2017.10.001. Epub 2017 Oct 12.
This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns' umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions.
Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background.
Cord blood GDNF levels were significantly higher (p=0.004) in preterm newborns (n=6) (115.05±57.17,pg/ml) when compared to term newborns (n=14) (19.67±10.67,pg/ml). GDNF levels in CSF trended (p=0.07) higher in term newborns (n=10) (19.56±9.11,pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n=5) (14.49±3.53,pg/ml).
GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.
本研究旨在调查不同围产期生长参数和临床状况下新生儿脐带血和脑脊液中胶质细胞源性神经营养因子（GDNF）的水平。
收集了20例新生儿的脐带血和58份残留脑脊液样本（临床检测完成后保存）。使用R&D Systems公司的GDNF ELISA试剂盒一式三份测定GDNF水平，并设置适当对照以消除背景干扰。
与足月儿（n = 14）（19.67±10.67，pg/ml）相比，早产儿（n = 6）（115.05±57.17，pg/ml）的脐带血GDNF水平显著更高（p = 0.004）。与足月或过期矫正胎龄的早产儿（n = 5）（14.49±3.53，pg/ml）相比，足月儿（n = 10）脑脊液中的GDNF水平呈上升趋势（p = 0.07）（19.56±9.11，pg/ml）。
与足月儿相比，早产儿脐带血中的GDNF水平更高，脑脊液中的GDNF水平更低。进一步研究不同胎龄和临床状况下新生儿的循环和脑脊液GDNF水平具有重要意义。
