Aix Marseille Univ, CNRS, ISM, Inst Movement Sci, Marseille, France.
Aix Marseille Univ, CNRS, ISM, Inst Movement Sci, Marseille, France; Assistance Publique-Hôpitaux de Marseille (APHM), Hôpital Timone, Service d'Odontologie, Marseille, France.
J Endod. 2017 Dec;43(12):2074-2080. doi: 10.1016/j.joen.2017.07.010. Epub 2017 Oct 9.
Numerous studies reported dentin bridge formation after pulp capping with tricalcium silicates. By contrast, pulp capping with resins leads to pulp toxicity and inflammation. Hybrid materials made up of tricalcium silicates and resins have also been developed to be used in direct pulp capping. This work was designed to study the consequences of adding resins to tricalcium silicates by investigating TheraCal (BISCO, Lançon De Provence, France) and Biodentine (Septodont, Saint Maur des Fosses, France) interactions with the dental pulp.
Media conditioned with the biomaterials were used to analyze pulp fibroblast proliferation using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) test and proinflammatory cytokine interleukin 8 (IL-8) secretion using the enzyme-linked immunosorbent assay. The effects of conditioned media on dentin sialoprotein (DSP) and nestin expression by dental pulp stem cells (DPSCs) were investigated by immunofluorescence. The materials' interactions with the vital pulp were investigated using the entire tooth culture model.
TheraCal-conditioned media significantly decreased pulp fibroblast proliferation, whereas no effect was observed with Biodentine. When DPSCs were cultured with Biodentine-conditioned media, immunofluorescence showed an increased expression of DSP and nestin. This expression was lower with TheraCal, which significantly induced proinflammatory IL-8 release both in cultured fibroblasts and entire tooth cultures. This IL-8 secretion increase was not observed with Biodentine. Entire tooth culture histology showed a higher mineralization with Biodentine, whereas significant tissue disorganization was observed with TheraCal.
Within the limits of these preclinical results, resin-containing TheraCal cannot be recommended for direct pulp capping.
许多研究报道了三钙硅酸钠盖髓后牙本质桥的形成。相比之下，树脂盖髓会导致牙髓毒性和炎症。为了在直接盖髓术中使用，还开发了由三钙硅酸钠和树脂组成的混合材料。本研究旨在通过研究 Theracal（BISCO，Lançon De Provence，法国）和 Biodentine（Septodont，Saint Maur des Fosses，法国）与牙髓的相互作用，研究向三钙硅酸钠中添加树脂的后果。
用生物材料调节培养基，通过 MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四唑溴盐）试验分析牙髓成纤维细胞增殖，通过酶联免疫吸附试验分析促炎细胞因子白细胞介素 8（IL-8）分泌。通过免疫荧光法研究条件培养基对牙髓干细胞（DPSCs）中牙本质涎蛋白（DSP）和巢蛋白表达的影响。通过全牙培养模型研究材料与活髓的相互作用。
Theracal 调节的培养基显著降低了牙髓成纤维细胞的增殖，而 Biodentine 则没有影响。当 DPSCs 与 Biodentine 调节的培养基共培养时，免疫荧光显示 DSP 和巢蛋白的表达增加。Theracal 的表达较低，它在培养的成纤维细胞和整个牙齿培养物中均显著诱导促炎细胞因子 IL-8 的释放。Biodentine 则没有观察到这种 IL-8 释放的增加。全牙培养组织学显示 Biodentine 具有更高的矿化，而 Theracal 则观察到明显的组织紊乱。
在这些临床前结果的限制范围内，含树脂的 Theracal 不能推荐用于直接盖髓。
