Department of Restorative Dentistry, Faculty of Dental Surgery, University of Malta, Msida, Malta; School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom.
Dental Materials Science, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
J Endod. 2018 Jul;44(7):1115-1120. doi: 10.1016/j.joen.2018.04.002. Epub 2018 Jun 1.
Pulp capping materials need to be able to protect the pulp but also bond to the overlying restorative materials. Light-curable pulp capping materials bond better to restorative materials and are easier to place than most water-based cements. The aim of this study was to characterize new light-curable tricalcium silicate-based pulp capping materials and compare their surface and antimicrobial properties with clinically available Theracal (Bisco, Schaumburg, IL) and Biodentine (Septodont, Saint-Maur-des-Fossés, France).
The surface characteristics of 3 light-curable pulp capping materials based on a resin and filled with tricalcium silicate and tantalum oxide radiopacifier and Theracal and Biodentine were assessed by scanning electron microscopy, X-ray diffraction, and contact angle measurement. The radiopacity was measured following ISO 6876 standards. The antimicrobial activity was determined by the direct contact test and the antibiofilm activity by the adenosine triphosphate assay and the confocal laser scanning Live/Dead assay (Invitrogen, Eugene, OR) using a polymicrobial culture.
The surface characteristics of the materials varied with the unfilled resin and Biodentine exhibiting a hydrophobic surface. Biodentine showed significantly higher antimicrobial properties in the direct contact test, but this property was absent in the antibiofilm activity tests. The resins filled with tricalcium silicate and Theracal showed higher antimicrobial activity than Biodentine in the adenosine triphosphate and live/dead assays.
The surface characteristics of a material affect its antimicrobial properties. The experimental resin-modified materials exhibited comparable antimicrobial properties with other light-curable pulp capping agents. Further long-term studies on the materials' antimicrobial activity are required to assess whether they can result in better clinical outcomes.
牙髓覆盖材料需要既能保护牙髓，又能与覆盖的修复材料粘结。光固化牙髓覆盖材料与修复材料的粘结性更好，且比大多数水基水泥更容易操作。本研究旨在对新型光固化硅酸三钙基牙髓覆盖材料进行特性分析，并与临床常用的Theracal（Bisco，Schaumburg，IL）和 Biodentine（Septodont，Saint-Maur-des-Fossés，法国）进行对比，分析其表面和抗菌性能。
通过扫描电子显微镜、X 射线衍射和接触角测量对 3 种基于树脂的光固化牙髓覆盖材料（填充硅酸三钙和氧化钽放射阻射剂）的表面特性进行评估，并与 Theracal 和 Biodentine 进行对比。根据 ISO 6876 标准测量放射阻射率。通过直接接触试验测定抗菌活性，通过三磷酸腺苷（ATP）测定和共聚焦激光扫描活/死试验（Invitrogen，Eugene，OR）测定抗生物膜活性，采用多微生物培养。
材料的表面特性因未填充树脂和 Biodentine 的疏水性而有所不同。Biodentine 在直接接触试验中表现出明显更高的抗菌性能，但在抗生物膜活性试验中则无此特性。填充硅酸三钙的树脂和 Theracal 在 ATP 和活/死试验中表现出比 Biodentine 更高的抗菌活性。
材料的表面特性会影响其抗菌性能。实验用树脂改性材料与其他光固化牙髓覆盖剂具有相当的抗菌性能。需要对这些材料的抗菌活性进行进一步的长期研究，以评估它们是否能带来更好的临床效果。
