Department of Orthodontics, The Institute of Craniofacial Deformity, College of Dentistry, Yonsei University, Seoul 03722, Republic of Korea.
Department and Research Institute of Dental Biomaterials and Bioengineering, BK21 PLUS Project, College of Dentistry, Yonsei University, Seoul 03722, Republic of Korea.
Dent Mater. 2017 Dec;33(12):1426-1435. doi: 10.1016/j.dental.2017.09.017. Epub 2017 Oct 13.
We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution, in low vacuum, and under ambient conditions after ultraviolet light (UV) treatment is comparable to that of the surface immediately after UV treatment for 15min and that after dielectric barrier discharge (DBD) plasma treatment for 15min.
Grade IV titanium discs with machined surfaces were irradiated with UV and their surface properties were evaluated immediately and after storage for 28days in distilled HO (dHO), a vacuum desiccator (31.325kPa), and a sealed container under air. Their surface characteristics were evaluated by atomic force microscopy, X-ray diffraction, contact angle analysis, and X-ray photoelectron spectroscopy. Biological activities were determined by analyzing the albumin adsorption, MC3T3-E1 cell adhesion, and cytoskeleton development.
Hydrophilicity of titanium surfaces stored in dHO was comparable to that immediately after UV treatment and higher than that immediately after DBD plasma treatment (P<0.001). Storage in dHO and in low vacuum immediately after UV treatment prevented hydrocarbon contamination and maintained elevated amounts of titanium and oxygen. After 28 days, protein adsorption, cellular adhesion, and cytoskeletal development of MC3T3-E1 cells on the titanium surfaces stored in dHO were significantly enhanced compared to those stored in low vacuum and under ambient conditions while being comparable to those immediately after UV and DBD plasma treatments.
UV treatment of the titanium implants followed by wet storage is useful for maintaining enhanced biological activity and overcoming biological aging during shelf storage.
我们评估了钛表面在低真空和环境条件下保存于水溶液中，经过紫外线（UV）处理后的生物学活性是否与 UV 处理 15 分钟后以及介质阻挡放电（DBD）等离子体处理 15 分钟后的表面的生物学活性相当。
用加工后的 IV 级钛盘进行紫外线照射，对其表面性能进行评估，然后在蒸馏水中（dHO）、真空干燥器（31.325kPa）和密封容器中于空气中储存 28 天，分别对其表面性能进行评估，评估方法为原子力显微镜、X 射线衍射、接触角分析和 X 射线光电子能谱。通过分析白蛋白吸附、MC3T3-E1 细胞黏附及细胞骨架发育，来确定生物活性。
储存在 dHO 中的钛表面的亲水性与 UV 处理后立即的亲水性相当，高于 DBD 等离子体处理后立即的亲水性（P<0.001）。储存在 dHO 和 UV 处理后立即的低真空中可以防止碳氢化合物污染，并保持钛和氧的含量升高。28 天后，储存在 dHO 中的钛表面的蛋白质吸附、细胞黏附和 MC3T3-E1 细胞骨架发育明显增强，与储存在低真空和环境条件下的钛表面相比，与 UV 和 DBD 等离子体处理后立即的钛表面相当。
紫外线处理钛植入物后进行湿存储有助于保持增强的生物活性，并克服在货架存储期间的生物老化。
