Ikewuchi Jude C, Uwakwe Augustine A, Onyeike Eugene N, Ikewuchi Catherine C
Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Nigeria.
EXCLI J. 2011 Dec 8;10:280-289. eCollection 2011.
The potential of aqueous extract of the leaves of , to protect against carbon tetrachloride induced liver damage was investigated in Wistar albino rats. The carbon tetrachloride was prepared 1:5 (v:v) in olive oil, and administered subcutaneously at 1 mL/kg body weight. The extract was administered to both normal and carbon tetrachloride treated rats at 100, 200 and 300 mg/kg. On fractionation and gas chromatographic analysis of the crude aqueous extract, thirty nine known alkaloids were detected, consisting mainly of akuamidine (69.027 %), voacangine (26.226 %), echitamine (1.974 %), echitamidine (0.599 %), lupanine (0.521 %) and augustamine (0.278 %). Compared to test control, the treatment dose dependently produced significantly lower (<0.05) alkaline phosphatase, aspartate and alanine transaminase activities. Histopathological studies on the liver sections showed that pre-treatment with the extract protected against carbon tetrachloride induced fatty degeneration of hepatocytes, thus, confirming the results of the biochemical studies. The above results imply that treatment with the plant extract protects the liver against carbon tetrachloride induced hepatotoxicity, therefore, justifying the use of in African traditional health care for the management of liver problems.
在Wistar白化大鼠中研究了[植物名称]叶水提取物预防四氯化碳诱导的肝损伤的潜力。四氯化碳按1:5（v:v）的比例用橄榄油配制，以1 mL/kg体重皮下注射。提取物以100、200和300 mg/kg的剂量分别给予正常大鼠和经四氯化碳处理的大鼠。对粗水提取物进行分馏和气相色谱分析后，检测到39种已知生物碱，主要包括阿枯米定（69.027%）、沃坎京（26.226%）、依西他明（1.974%）、依西他米定（0.599%）、羽扇豆碱（0.521%）和奥古斯塔明（0.278%）。与试验对照组相比，治疗组剂量依赖性地显著降低（<0.05）碱性磷酸酶、天冬氨酸和丙氨酸转氨酶活性。肝脏切片的组织病理学研究表明，提取物预处理可预防四氯化碳诱导的肝细胞脂肪变性，并证实了生化研究结果。上述结果表明，用该植物提取物治疗可保护肝脏免受四氯化碳诱导的肝毒性，因此，这也证明了在非洲传统医疗中使用[植物名称]治疗肝脏问题的合理性。
