Zhang J, Cui L, Ma J, Chen C, Yang B, Huang L
State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, 330045, Nanchang, China.
Anim Genet. 2017 Dec;48(6):645-652. doi: 10.1111/age.12597. Epub 2017 Oct 16.
Fatty acid composition is associated with meat quality in pigs as well as with obesity- and diabetes-related traits in humans. Liver and muscle are important tissues for fatty acid metabolism. In this study, we evaluated correlations between liver and muscle transcriptomes and fatty acid composition traits in muscle and abdominal fat tissues in 335 F pigs from a White Duroc × Erhualian F pig resource population. Transcripts significantly correlated with fatty acid composition traits were enriched for genes involved in the categories of triglyceride catabolic process, mitochondrial function, hematological and immune system, and disease such as Type II diabetes. Gene co-expression network analysis further identified liver network modules relevant to fatty acid unsaturation index that were enriched in platelet activation and the type I interferon signaling pathway and highlighted the connections between variations in fatty acid composition and genes involved in hematological and immune system. Integrative analysis of the expression QTL identified ELOVL6 and SCD as plausible candidate genes underlying the loci for muscle C18:1n-9/C16:1n-7 values on chromosome 8 and the loci for muscle C18:1n-9/C18:0 contents on chromosome 14 respectively.
脂肪酸组成与猪的肉质以及人类与肥胖和糖尿病相关的性状有关。肝脏和肌肉是脂肪酸代谢的重要组织。在本研究中，我们评估了来自大白杜洛克×二花脸F猪资源群体的335头F猪的肝脏和肌肉转录组与肌肉和腹部脂肪组织中脂肪酸组成性状之间的相关性。与脂肪酸组成性状显著相关的转录本在参与甘油三酯分解代谢过程、线粒体功能、血液和免疫系统以及II型糖尿病等疾病的基因中富集。基因共表达网络分析进一步确定了与脂肪酸不饱和度指数相关的肝脏网络模块，这些模块在血小板活化和I型干扰素信号通路中富集，并突出了脂肪酸组成变化与血液和免疫系统相关基因之间的联系。表达QTL的综合分析分别确定ELOVL6和SCD是8号染色体上肌肉C18:1n-9/C16:1n-7值位点和14号染色体上肌肉C18:1n-9/C18:0含量位点的潜在候选基因。
