Hwang David, Fong Hanson, Johnson James D, Paranjpe Avina
Department of Endodontics, University of Washington, Seattle, Washington, USA.
Department of Material Science and Engineering, University of Washington, Seattle, Washington, USA.
Aust Endod J. 2018 Dec;44(3):208-214. doi: 10.1111/aej.12228. Epub 2017 Oct 16.
Chemical debridement during Regenerative Endodontic procedures is important. Previous research studies have evaluated various antibiotics and their concentrations but none have addressed the concern of delivering these materials. Hence, the purpose of this study was to determine what carrier could be used effectively in a clinical setting. Sixty caries-free maxillary incisors were used and inoculated with Enterococcus faecalis and divided into positive and irrigation controls and experimental groups that had triple antibiotic powder (1:1:1 ciprofloxacin:metronidazole:minocycline) delivered using various carriers: saline, cotton, sponge and methylcellulose. Current AAE regenerative protocols were followed. S2 sampling was performed and tested for bacterial presence via culturing and SEM. The results demonstrated that saline was the most effective carrier for the triple antibiotic powder while cotton and sponge were most ineffective. Saline and methylcellulose both reduced bacterial counts to a significant level. Overall, this study demonstrated that saline as a carrier was most effective and should be routinely used.
在牙髓再生治疗过程中进行化学清创很重要。以往的研究评估了各种抗生素及其浓度，但没有一项研究涉及这些材料的输送问题。因此，本研究的目的是确定在临床环境中可以有效使用哪种载体。使用了60颗无龋的上颌切牙，接种粪肠球菌后分为阳性对照组、冲洗对照组和实验组，实验组使用各种载体输送三联抗生素粉末（环丙沙星：甲硝唑：米诺环素，比例为1:1:1）：生理盐水、棉花、海绵和甲基纤维素。遵循当前美国牙髓病协会的牙髓再生方案。进行S2采样，并通过培养和扫描电子显微镜检测细菌的存在情况。结果表明，生理盐水是输送三联抗生素粉末最有效的载体，而棉花和海绵最无效。生理盐水和甲基纤维素都将细菌数量显著降低。总体而言，本研究表明生理盐水作为载体最有效，应常规使用。
