Zhang Fei, Bai Fengwu, Zhao Xinqing
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Sheng Wu Gong Cheng Xue Bao. 2016 Nov 25;32(11):1481-1495. doi: 10.13345/j.cjb.160105.
Production of bioenergy and bio-based chemicals by using fermentable sugars released from low-cost renewable lignocellulosic biomass has received great attention. Efficient cellulolytic enzymes are crucial for lignocellulose bioconversion, but high cellulase production cost is limiting the bioconversion efficiency of cellulosic biomass and industrial applications of lignocellulose biorefinery. Studies on induction and regulation of cellulase in filamentous fungi will help to further develop superior fungal strains for efficient cellulase production and reduce cellulase production cost. With the advances in high-throughput sequencing and gene manipulation technology using fungal strains, an in-depth understanding of cellulase induction and regulation mechanisms of enzyme expression has been achieved. We reviewed recent progresses in the induction and regulation of cellulase expression in several model filamentous fungi, emphasizing sugar transporters, transcription factors and chromatin remodeling. Future prospects in application of artificial zinc finger proteins for cellulase induction and regulation in filamentous fungi were discussed.
利用低成本可再生木质纤维素生物质释放的可发酵糖生产生物能源和生物基化学品已受到广泛关注。高效的纤维素分解酶对于木质纤维素生物转化至关重要，但高纤维素酶生产成本限制了纤维素生物质的生物转化效率以及木质纤维素生物精炼的工业应用。对丝状真菌中纤维素酶的诱导和调控进行研究，将有助于进一步开发高效生产纤维素酶的优良真菌菌株，并降低纤维素酶生产成本。随着高通量测序和利用真菌菌株的基因操作技术的进步，人们已深入了解了纤维素酶诱导和酶表达调控机制。我们综述了几种模式丝状真菌中纤维素酶表达诱导和调控的最新进展，重点关注糖转运蛋白、转录因子和染色质重塑。讨论了人工锌指蛋白在丝状真菌纤维素酶诱导和调控中的应用前景。
