碱性成纤维细胞生长因子对雄性叙利亚仓鼠辐射诱导的口腔黏膜炎的影响。
Effect of basic fibroblast growth factor on radiation-induced oral mucositis in male Syrian hamsters.
作者信息
Sumikawa Satomi, Watanabe Shinichi, Tanaka Mamoru, Tanaka Akihiro, Araki Hiroaki
机构信息
a Division of Pharmacy , Ehime University Hospital , Toon , Japan.
出版信息
Int J Radiat Biol. 2017 Dec;93(12):1343-1349. doi: 10.1080/09553002.2017.1393578. Epub 2017 Nov 6.
PURPOSE
Oral mucositis is a frequent and dose-limiting side effect of radiotherapy or chemotherapy in cancer patients. We investigated the effect of basic fibroblast growth factor on radiation-induced oral mucositis in male Syrian hamsters.
METHOD
Oral mucositis was induced in male Syrian hamsters by a single dose of 30-Gy irradiation. Eight days after irradiation, treatment with gel containing trafermin (basic fibroblast growth factor) at 1 or 10 μg up to day-21 was initiated. Re-epithelialization was graded using a six-point scoring system for oral mucositis. Samples of hamster cheek pouches were removed for histopathologic analyses and immunohistochemistry.
RESULTS
The oral-mucositis score decreased in a dose-dependent manner upon trafermin treatment. Trafermin (10 μg) improved oral mucositis significantly compared with vehicle. Histopathology revealed that the degree of re-epithelialization was improved by treatment with trafermin (10 μg) compared with treatment with vehicle.
CONCLUSIONS
Administration of trafermin (10 μg) can prevent mucosal damage to hamster cheek pouches induced by irradiation.
目的
口腔黏膜炎是癌症患者放疗或化疗常见的剂量限制性副作用。我们研究了碱性成纤维细胞生长因子对雄性叙利亚仓鼠放射性口腔黏膜炎的影响。
方法
对雄性叙利亚仓鼠单次给予30 Gy照射以诱导口腔黏膜炎。照射8天后，开始用含1或10μg曲弗明（碱性成纤维细胞生长因子）的凝胶进行治疗，直至第21天。使用口腔黏膜炎六点评分系统对再上皮化进行分级。取仓鼠颊囊样本进行组织病理学分析和免疫组织化学检查。
结果
曲弗明治疗后口腔黏膜炎评分呈剂量依赖性降低。与赋形剂相比，曲弗明（10μg）显著改善了口腔黏膜炎。组织病理学显示，与赋形剂治疗相比，曲弗明（10μg）治疗改善了再上皮化程度。
结论
给予曲弗明（10μg）可预防照射对仓鼠颊囊造成的黏膜损伤。
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