Ma Guo-Quan, Xia Zhan-Feng, Wan Chuan-Xing, Zhang Yao, Luo Xiao-Xia, Zhang Li-Li
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps; Tarim University, Alar 843300, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):4892-4896. doi: 10.1099/ijsem.0.002125. Epub 2017 Oct 16.
A novel actinobacterium designated TRM 46509 was isolated from a salt water beach at Kalpin, Xinjiang, north-west China. The strain was aerobic and Gram-stain-positive, with an optimum NaCl concentration for growth of 1 % (w/v). The isolate formed sparse aerial mycelium and produced spiral spores at the end of the aerial mycelium on Gauze's No. 1 medium. The isolate contained ll-diaminopimelic acid as the diagnostic diamino acid and ribose as the major whole-cell sugar. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were MK-9(H2), MK-9(H6) and MK-9(H8). The major fatty acids were C16:0, iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0 and iso-C14 : 0. The G+C content of the DNA was 69.3 mol%. Phylogenetic analysis showed that strain TRM 46509 shared 16S rRNA gene sequence similarity of 97.6 % with the closest described species Streptomyces tacrolimicus ATCC 55098. On the basis of evidence from this polyphasic study, strain TRM 46509 should be designated as representing a novel species of the genus Streptomyces, for which the name Streptomyces kalpinensis sp. nov. is proposed. The type strain is TRM 46509 (=CCTCC AA 2015028=KCTC 39667).
从中国西北部新疆卡尔平的一个咸水海滩分离出一株新的放线菌，命名为TRM 46509。该菌株为需氧菌，革兰氏染色阳性，生长的最适NaCl浓度为1%（w/v）。在一号纱布培养基上，该分离株形成稀疏的气生菌丝体，并在气生菌丝体末端产生螺旋孢子。该分离株含有ll-二氨基庚二酸作为诊断性二氨基酸，核糖作为主要的全细胞糖。极性脂质包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂。主要的甲基萘醌是MK-9(H2)、MK-9(H6)和MK-9(H8)。主要脂肪酸为C16:0、异-C16:0、anteiso-C15:0、异-C15:0和异-C14:0。DNA的G+C含量为69.3mol%。系统发育分析表明，菌株TRM 46509与最接近的已描述物种链霉菌属的他克莫司链霉菌ATCC 55098的16S rRNA基因序列相似性为97.6%。基于这项多相研究的证据，菌株TRM 46509应被指定为代表链霉菌属的一个新物种，为此提出名为卡尔平链霉菌新种（Streptomyces kalpinensis sp. nov.）。模式菌株为TRM 46509（=CCTCC AA 2015028=KCTC 39667）。
