Wang Zhikuan, Jiang Binjin, Li Xiaoguang, Gan Longzhan, Long Xiufeng, Zhang Yuqin, Tian Yongqiang
1Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
2Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2568-2573. doi: 10.1099/ijsem.0.002877. Epub 2018 Jun 26.
A novel endophytic actinobacterium strain, designated A249, was isolated from the stem of Populus adenopoda collected at Mount Qingcheng in south-west China. Its taxonomic position was determined by using a polyphasic approach. The cultural and morphological characteristics of isolate A249 were consistent with members of the genus Streptomyces. Growth occurred at 10-37 °C, pH 6.0-12.0 and in the presence of 0-4 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence and phylogenetic trees showed the closest phylogenetic relatives to strain A249 were Streptomyces shaanxiensis JCM 16925 (98.0 % 16S rRNA gene sequence similarity) and Streptomyces lanatus JCM 4332 (97.9 %). The DNA-DNA hybridization values between the strain A249 and the two reference strains ranged from 41.4 to 49.4 %. The DNA G+C content was 71.7 mol%. The range of average nucleotide identity values was 81.5-86.7 %. Chemical analysis of cellular components indicated that strain A249 contained ll-diaminopimelic acid, xylose and galactose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipids were diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylethanolamine, two unidentified lipids, one unidentified phospholipid, one unidentified aminolipid and one unidentified aminophospholipid. The major fatty acids comprised C16 : 0, iso-C14 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and C16 : 1ω7c. On the basis of the phenotypic and genotypic differentiation of the three tested strains, isolate A249 is proposed to represent a novel species of the genus Streptomyces, named Streptomyces populi sp. nov. The type strain is A249 (=CGMCC 4.7417=JCM 32175).
从中国西南部青城山采集的响叶杨茎中分离出一株新型内生放线菌菌株，命名为A249。采用多相分类法确定其分类地位。分离株A249的培养和形态特征与链霉菌属成员一致。在10-37 °C、pH 6.0-12.0以及存在0-4 %（w/v）NaCl的条件下均可生长。16S rRNA基因序列分析和系统发育树显示，与菌株A249亲缘关系最近的是陕西链霉菌JCM 16925（16S rRNA基因序列相似性为98.0 %）和羊毛链霉菌JCM 4332（97.9 %）。菌株A249与两个参考菌株之间的DNA-DNA杂交值在41.4%至49.4 %之间。DNA G+C含量为71.7 mol%。平均核苷酸同一性值范围为81.5-86.7 %。细胞成分的化学分析表明，菌株A249含有ll-二氨基庚二酸、木糖和半乳糖。主要的甲基萘醌是MK-9(H6)和MK-9(H8)。极性脂质为双磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰乙醇胺、两种未鉴定的脂质、一种未鉴定的磷脂、一种未鉴定的氨基脂质和一种未鉴定的氨基磷脂。主要脂肪酸包括C16 : 0、异-C14 : 0、异-C15 : 0、异-C16 : 0、anteiso-C15 : 0和C16 : 1ω7c。基于对三个测试菌株的表型和基因型差异，提议分离株A249代表链霉菌属的一个新物种，命名为杨树链霉菌（Streptomyces populi sp. nov.）。模式菌株为A249（=CGMCC 4.7417=JCM 32175）。
