Wang Jianming, Zhang Qiaoyan, Wang Yang, Qin Song, Luo Xiaoxia
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Int J Syst Evol Microbiol. 2018 Jul;68(7):2178-2182. doi: 10.1099/ijsem.0.002804. Epub 2018 May 31.
A novel actinobacterium, designated TRM46399, was isolated from ditch sediment in Xinjiang Province, Northwest China. Phylogenetic, physiological, chemotaxonomic and morphological analyses demonstrated that strain TRM46399 belonged to the genus Streptomyces. The strain was aerobic, Gram-stain-positive, had aerial mycelia that branched monopodially and formed chains of arthrospores. The spores were oval to cylindrical with smooth surfaces. The strain had an optimum NaCl concentration for growth of 5 % (w/v). The whole-cell sugar pattern of TRM46399 consisted of ribose, xylose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H10). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids were iso-C16 : 0 (47.8 %), iso-C14 : 0 (13.5 %), C16 : 0 (8.9 %), anteiso-C15 : 0 (8.4 %), anteiso-C17 : 0 (4.2 %) and C17 : 0cyclo (2.7 %). The G+C content of the genomic DNA was 68.7 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain TRM46399 (KY688182) was phylogenetically most closely related to Streptomyces xinghaiensis S187 (99.13 % sequence similarity); however, DNA-DNA hybridization studies between stain TRM46399 and S. xinhaiensis S187 showed only 65.32 % relatedness. Based on the evidence from this polyphasic study, strain TRM46399 represents a novel species of the genus Streptomyces, for which the name Streptomyces aqsuensis sp. nov. is proposed. The type strain is TRM46399 (=KCTC 39610 =CCTCC AA 2015009).
从中国西北新疆地区的沟渠沉积物中分离出一株新型放线菌，命名为TRM46399。系统发育、生理、化学分类和形态学分析表明，菌株TRM46399属于链霉菌属。该菌株为需氧菌，革兰氏染色阳性，有气生菌丝，气生菌丝单轴分枝并形成节孢子链。孢子呈椭圆形至圆柱形，表面光滑。该菌株生长的最适NaCl浓度为5%（w/v）。TRM46399的全细胞糖型由核糖、木糖、甘露糖和半乳糖组成。主要的甲基萘醌为MK-9(H6)、MK-9(H8)和MK-9(H10)。极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要脂肪酸为异-C16 : 0（47.8%）、异-C14 : 0（13.5%）、C16 : 0（8.9%）、anteiso-C15 : 0（8.4%）、anteiso-C17 : 0（4.2%）和C17 : 0环（2.7%）。基因组DNA的G+C含量为68.7mol%。比较16S rRNA基因序列分析表明，菌株TRM46399（KY688182）在系统发育上与星海链霉菌S187关系最密切（序列相似性为99.13%）；然而，菌株TRM46399与星海链霉菌S187之间的DNA-DNA杂交研究显示它们之间的相关性仅为65.32%。基于这项多相研究的证据，菌株TRM46399代表链霉菌属的一个新物种，为此提出新名称阿克苏链霉菌（Streptomyces aqsuensis sp. nov.）。模式菌株为TRM46399（=KCTC 39610 =CCTCC AA 2015009）。
