Chakritbudsabong Warunya, Sariya Ladawan, Pamonsupornvichit Sirikron, Pronarkngver Rassmeepen, Chaiwattanarungruengpaisan Somjit, Ferreira Joao N, Setthawong Piyathip, Phakdeedindan Praopilas, Techakumphu Mongkol, Tharasanit Theerawat, Rungarunlert Sasitorn
Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MOZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Stem Cell Res. 2017 Oct;24:21-24. doi: 10.1016/j.scr.2017.08.005. Epub 2017 Aug 5.
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.
猪诱导多能干细胞（piPSC）系是利用携带人类转录因子OCT4、SOX2、KLF4、c-MYC和LIN28的逆转录病毒转导方法，从胚胎成纤维细胞中产生的。所产生的piPSC系VSMUi001-D碱性磷酸酶活性呈阳性，并表达多能性相关转录因子，包括OCT4、SOX2、NANOG以及表面标志物SSEA-1，这些都是诱导多能干细胞真实性的所有标志性特征。此外，VSMUi001-D表现出正常的核型，在体外形成胚状体，在体内形成畸胎瘤。在心脏分化时，VSMUi001-D表现出自发搏动并表达心肌细胞标志物，如心肌肌钙蛋白T。
