Fang Fang, Li Zili, Zhao Qian, Ye Zhen, Zhao Kai, Gu Xiuli, Shen Shiliang, Reijo Pera Renee A, Xiong Chengliang
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Wuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, China.
Stem Cell Res. 2018 Mar;27:136-139. doi: 10.1016/j.scr.2018.01.026. Epub 2018 Jan 28.
Human fibroblasts were isolated from foreskin of a clinically diagnosed 40-year old patient with idiopathic infertility. The fibroblasts were reprogrammed with the Yamanaka KOSM transcriptional factors using the retroviral vectors. The obtained induced pluripotent stem cell (iPSC) line showed pluripotency verified by the expression of pluripotency markers, NANOG, SOX2, OCT4, TRA-1-60, and SSEA-4. And the iPSC line was demonstrated to have the three germ layers differentiation capacity in vivo by teratoma assay. The iPSC line also showed normal karyotype. This patient-specific iPSC line can be used to explore the mechanism for idiopathic male infertility.
人成纤维细胞取自一名临床诊断为特发性不育的40岁患者的包皮。使用逆转录病毒载体，用山中伸弥KOSM转录因子对成纤维细胞进行重编程。获得的诱导多能干细胞（iPSC）系通过多能性标志物NANOG、SOX2、OCT4、TRA-1-60和SSEA-4的表达验证了其多能性。并且通过畸胎瘤试验证明该iPSC系在体内具有三胚层分化能力。该iPSC系还显示出正常的核型。这种患者特异性iPSC系可用于探索特发性男性不育的机制。
