Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea.
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea.
Mol Nutr Food Res. 2018 Feb;62(3). doi: 10.1002/mnfr.201700586. Epub 2017 Dec 14.
Black rice extract (BRE) contains cyanidin 3-O-glucoside (C3G), an anthocyanin, as the major component. In this study, we found that BRE inhibits the mRNA and protein expression of genes encoding cytotoxin-associated protein A (cagA) and vacuolating protein A (vacA) in Helicobacter pylori 60190 strain.
We performed RT-PCR and western blotting to show that BRE inhibits the mRNA and protein expression of SecA. Because SecA is involved in VacA export in bacteria, our result suggests a positive correlation between BRE-induced inhibition of secA expression and VacA secretion. Further, we perform MTT assay and flow cytometry to show that BRE decreases the apoptosis of H. pylori-infected KATO III cells. Finally, we perform western blotting to show that the cell-protective effect of BRE is associated with decreased levels of active proapoptotic proteins caspases and PARP and increased levels of antiapoptotic proteins survivin and XIAP in H. pylori-infected cells.
Thus, our results indicate that BRE acts as a potent inhibitor of the biogenesis of H. pylori virulence proteins and decreases the apoptosis of H. pylori-infected cells. Moreover, our results suggest that BRE can be used to exert beneficial effects in patients with gastroduodenal diseases caused by H. pylori.
黑米提取物（BRE）含有作为主要成分的矢车菊素 3-O-葡萄糖苷（C3G），一种花色苷。在这项研究中，我们发现 BRE 抑制了幽门螺杆菌 60190 株细胞毒素相关蛋白 A（cagA）和空泡形成蛋白 A（vacA）编码基因的 mRNA 和蛋白质表达。
我们进行了 RT-PCR 和 Western blot 实验，结果表明 BRE 抑制了 SecA 的 mRNA 和蛋白质表达。由于 SecA 参与细菌中 VacA 的输出，我们的结果表明 BRE 诱导的 secA 表达抑制与 VacA 分泌之间存在正相关。此外，我们进行了 MTT 测定和流式细胞术实验，结果表明 BRE 减少了幽门螺杆菌感染的 KATO III 细胞的凋亡。最后，我们进行了 Western blot 实验，结果表明 BRE 的细胞保护作用与幽门螺杆菌感染细胞中活性促凋亡蛋白 caspase 和 PARP 的水平降低以及抗凋亡蛋白 survivin 和 XIAP 的水平升高有关。
因此，我们的结果表明 BRE 作为一种有效的幽门螺杆菌毒力蛋白生物发生抑制剂发挥作用，并减少了幽门螺杆菌感染细胞的凋亡。此外，我们的结果表明，BRE 可用于发挥对由幽门螺杆菌引起的胃十二指肠疾病患者的有益作用。
