a College of Life Science and Technology , Southwest University for Nationalities , Chengdu , P. R. China.
Anim Biotechnol. 2018 Jul 3;29(3):234-240. doi: 10.1080/10495398.2017.1369096. Epub 2017 Oct 16.
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca binding EF hand and could interact with actin filament. Its transcript was found in all tested tissues including spleen, brain, heart, kidney, liver, thymus, bursa of Fabricius, lung, and a relative low-level expression was detected in leg muscle. Furthermore, AIF1L expression in peripheral blood lymphocyte was depressed in a dose-dependent manner with cadmium exposure and peripheral blood lymphocyte viability decrease displayed a similar pattern with AIF1L expression. The results indicated that newly identified chicken AIF1L might be associated with lymphocyte viability.
同种异体炎症因子-1（AIF-1）是一种与炎症相关的蛋白，主要由免疫细胞产生，如单核细胞/巨噬细胞和活化的 T 淋巴细胞。它对于免疫细胞的存活和促炎活性至关重要。然而，AIF-1 在鸡中的功能尚未确定。在本研究中，在临朐鸡肉鸡中鉴定出 AIF-1 样（AIF1L）基因。生物信息学分析表明，鸡 AIF-1 蛋白的分子量为 16290.8 Da。AIF1L 含有一个 Ca 结合 EF 手，能够与肌动蛋白丝相互作用。其转录本在所有检测到的组织中均有表达，包括脾、脑、心、肾、肝、胸腺、法氏囊、肺，在腿部肌肉中表达水平相对较低。此外，随着镉暴露，外周血淋巴细胞中 AIF1L 的表达呈剂量依赖性下降，而外周血淋巴细胞活力下降与 AIF1L 表达呈现相似的模式。结果表明，新鉴定的鸡 AIF1L 可能与淋巴细胞活力有关。
