Chongqing Academy of Animal Husbandry, Chongqing 400039, China.
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China.
Molecules. 2017 Oct 16;22(10):1734. doi: 10.3390/molecules22101734.
Mats is an agronomically and economically important perennial legume shrub with a high forage yield, protein content and strong adaptability, which is subject to natural habitat fragmentation and serious human disturbance. Until now, our knowledge of the genetic relationships and intraspecific genetic diversity for its wild collections is still poor, especially at small spatial scales. Here amplified fragment length polymorphism (AFLP) technology was employed for analysis of genetic diversity, differentiation, and structure of 364 genotypes of from 15 natural locations in Wushan Montain, a highly structured mountain with typical karst landforms in Southwest China. We also tested whether eco-climate factors has affected genetic structure by correlating genetic diversity with habitat features. A total of 515 distinctly scoreable bands were generated, and 324 of them were polymorphic. The polymorphic information content (PIC) ranged from 0.694 to 0.890 with an average of 0.789 per primer pair. On species level, Nei's gene diversity (), the Bayesian genetic diversity index () and the Shannon information index () were 0.2465, 0.2363 and 0.3772, respectively. The high differentiation among all sampling sites was detected ( = 0.2217, = 0.1746, = 0.2060, = 0.1844), and instead, gene flow among accessions ( = 1.1819) was restricted. The population genetic structure resolved by the UPGMA tree, principal coordinate analysis, and Bayesian-based cluster analyses irrefutably grouped all accessions into two distinct clusters, i.e., lowland and highland groups. The population genetic structure resolved by the UPGMA tree, principal coordinate analysis, and Bayesian-based cluster analyses irrefutably grouped all accessions into two distinct clusters, i.e., lowland and highland groups. This structure pattern may indicate joint effects by the neutral evolution and natural selection. Restricted was observed across all accessions, and genetic barriers were detected between adjacent accessions due to specifically geographical landform.
刺槐是一种具有重要经济和农业价值的多年生豆科灌木，具有较高的饲料产量、蛋白质含量和较强的适应性，但它容易受到自然生境破碎化和人类严重干扰的影响。到目前为止，我们对其野生种群的遗传关系和种内遗传多样性的了解仍然很差，特别是在小空间尺度上。在这里，我们采用扩增片段长度多态性（AFLP）技术分析了来自中国西南典型喀斯特地貌的巫山山脉 15 个自然地点的 364 个 野生型的遗传多样性、分化和结构。我们还通过将遗传多样性与生境特征相关联，检验了生态气候因素是否影响了遗传结构。共产生了 515 个可明显评分的条带，其中 324 个为多态性。多态信息含量（PIC）范围为 0.694 至 0.890，平均每个引物对为 0.789。在种水平上，Nei 基因多样性（）、贝叶斯遗传多样性指数（）和香农信息指数（）分别为 0.2465、0.2363 和 0.3772。所有采样点之间均存在高度分化（=0.2217、=0.1746、=0.2060、=0.1844），而居群间的基因流（=1.1819）受到限制。UPGMA 树、主坐标分析和基于贝叶斯的聚类分析所解析的种群遗传结构毫不含糊地将所有居群分为两个不同的集群，即低地和高地集群。这种结构模式可能表明中性进化和自然选择的共同作用。在所有居群中都观察到了 的限制，由于特定的地理地貌，在相邻居群之间检测到了遗传障碍。
