Chen Yi-Tien, Lin Chao-Fen, Chen Young-Mao, Lo Chih-En, Chen Wan-Erh, Chen Tzong-Yueh
Laboratory of Molecular Genetics, Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
PLoS One. 2017 Oct 16;12(10):e0186506. doi: 10.1371/journal.pone.0186506. eCollection 2017.
Myostatin is a negative regulator of myogenesis and has been suggested to be an important factor in the development of muscle wasting during viral infection. The objective of this study was to characterize the main regulatory element of the grouper myostatin promoter and to study changes in promoter activity due to viral stimulation. In vitro and in vivo experiments indicated that the E-box E6 is a positive cis-and trans-regulation motif, and an essential binding site for MyoD. In contrast, the E-box E5 is a dominant negative cis-regulatory. The characteristics of grouper myostatin promoter are similar in regulation of muscle growth to that of other species, but mainly through specific regulatory elements. According to these results, we conducted a study to investigate the effect of viral infection on myostatin promoter activity and its regulation. The nervous necrosis virus (NNV) treatment significantly induced myostatin promoter activity. The present study is the first report describing that specific myostatin motifs regulate promoter activity and response to viral infection.
肌肉生长抑制素是肌生成的负调节因子，被认为是病毒感染期间肌肉萎缩发展的一个重要因素。本研究的目的是鉴定石斑鱼肌肉生长抑制素启动子的主要调控元件，并研究病毒刺激引起的启动子活性变化。体外和体内实验表明，E盒E6是一个正向顺式和反式调节基序，是MyoD的一个必需结合位点。相比之下，E盒E5是一个显性负向顺式调节元件。石斑鱼肌肉生长抑制素启动子在肌肉生长调节方面的特征与其他物种相似，但主要通过特定的调控元件。根据这些结果，我们进行了一项研究，以调查病毒感染对肌肉生长抑制素启动子活性及其调控的影响。神经坏死病毒（NNV）处理显著诱导了肌肉生长抑制素启动子活性。本研究是第一篇描述特定肌肉生长抑制素基序调节启动子活性并对病毒感染作出反应的报告。
