Liu Jun-Ying, Fan Hui-Yan, Wang Ying, Zhang Yong-Liang, Li Da-Wei, Yu Jia-Lin, Han Cheng-Gui
State Key Laboratory for Agro-Biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, P. R. China.
College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, China.
PLoS One. 2017 Oct 16;12(10):e0186500. doi: 10.1371/journal.pone.0186500. eCollection 2017.
Plant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylogenetic analysis was performed, and 8 Beta lineage-specific miRNAs were identified. Through a differential expression analysis, miRNAs associated with Beet necrotic yellow vein virus (BNYVV) infection were identified and confirmed using a microarray analysis and stem-loop RT-qPCR. In total, 103 known miRNAs representing 38 miRNA families, and 45 potential novel miRNAs were differentially regulated, with at least a two-fold change, in BNYVV-infected plants compared with that of the mock-inoculated control. Targets of these differentially expressed miRNAs were also predicted by degradome sequencing. These differentially expressed miRNAs were involved in hormone biosynthesis and signal transduction pathways, and enhanced axillary bud development and plant defenses. This work is the first to describe miRNAs of the plant genus Beta and may offer a reference for miRNA research in other species in the genus. It provides valuable information on the pathogenicity mechanisms of BNYVV.
植物微小RNA（miRNA）是一类非编码RNA，在植物发育、防御及症状发展中发挥重要作用。在此，利用小RNA深度测序在大果甜菜中鉴定出代表129个miRNA家族的547个已知miRNA以及282个潜在的新miRNA。进行了系统发育分析，鉴定出8个甜菜谱系特异性miRNA。通过差异表达分析，鉴定出与甜菜坏死黄脉病毒（BNYVV）感染相关的miRNA，并使用微阵列分析和茎环RT-qPCR进行了验证。与模拟接种对照相比，在受BNYVV感染的植物中，共有代表38个miRNA家族的103个已知miRNA和45个潜在的新miRNA受到差异调节，变化倍数至少为两倍。还通过降解组测序预测了这些差异表达miRNA的靶标。这些差异表达的miRNA参与激素生物合成和信号转导途径，并促进腋芽发育和植物防御。这项工作首次描述了甜菜属植物的miRNA，可能为该属其他物种的miRNA研究提供参考。它为BNYVV的致病机制提供了有价值的信息。
