Department of Oncology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
Department of Oncology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.
Cancer Biomark. 2017 Dec 12;21(1):23-28. doi: 10.3233/CBM-170172.
A recent study has demonstrated that the long non-coding RNA VPS9D1-AS1 is highly expressed in colorectal cancer and predicts poor prognosis. However, roles of VPS9D1-AS1 in gastric cancer remained poorly understood.
The aim of this study is to decipher the expression of VPS9D1-AS1 in gastric cancer (GC) patients, so as to assess whether or not it could be used as a novel biomarker for prognosis in gastric cancer patients.
The expression of VPS9D1-AS1 was examined in cancer tissues and paired adjacent non-tumorous tissues from 126 gastric cancer patients using qRT-PCR. Correlations between the expression of VPS9D1-AS1 and clinicopathological parameters and patients' survival were analyzed.
VPS9D1-AS1 expression was downregulated in gastric cancer tissues than that in adjacent non-tumorous tissues (P< 0.001). VPS9D1-AS1 expression level was markedly correlated with tumor size and TNM stage in gastric cancer. Kaplan-Meier analysis showed low expression of VPS9D1-AS1 were correlated with poor overall and disease free survival. On multivariate analysis, the hazard ratio of VPS9D1-AS expression was 0.30 (95% CI = 0.13-0.66, P= 0.003) for overall survival.
Overall, our data suggest that downregulated VPS9D1-AS1 may be used as a novel prognosis predictor of gastric cancer.
最近的一项研究表明，长非编码 RNA VPS9D1-AS1 在结直肠癌中高度表达，并预测预后不良。然而，VPS9D1-AS1 在胃癌中的作用仍知之甚少。
本研究旨在破译胃癌患者中 VPS9D1-AS1 的表达，以评估其是否可作为胃癌患者预后的新型生物标志物。
采用 qRT-PCR 检测 126 例胃癌患者癌组织及配对癌旁非肿瘤组织中 VPS9D1-AS1 的表达。分析 VPS9D1-AS1 的表达与临床病理参数及患者生存的相关性。
VPS9D1-AS1 在胃癌组织中的表达低于癌旁非肿瘤组织（P<0.001）。VPS9D1-AS1 的表达水平与胃癌的肿瘤大小和 TNM 分期显著相关。Kaplan-Meier 分析显示 VPS9D1-AS1 低表达与总生存期和无病生存期不良相关。多因素分析显示，VPS9D1-AS 表达的危险比为 0.30（95%CI=0.13-0.66，P=0.003）。
总体而言，我们的数据表明下调的 VPS9D1-AS1 可能作为胃癌的一种新的预后预测因子。
