Shi X-Y, Sun Y-Z, Li M, Li H-Y
Department of Laboratory Medicine, The Children and Women's Healthcare of Laiwu City, Laiwu, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):232-238. doi: 10.26355/eurrev_201908_18652.
To investigate the expression of the long non-coding RNA CADM1-AS1 (lncRNA CADM1-AS1) in gastric cancer and its clinical significance.
The real time fluorescence quantitative polymerase chain reaction (qRT-PCR) was performed to detect the expression difference of lncRNA CADM1-AS1 between gastric cancer and its adjacent normal tissues. Then, the correlation between the expression of CADM1-AS1 in gastric cancer and the clinicopathological characteristics was analyzed by the Chi-square test. Moreover, the potential of lncRNA CADM1-AS1 in predicting the prognosis of patients with GC after the operation was assessed by the Log-rank test and the Cox's proportional hazards regression model.
The expression of lncRNA CADM1-AS1 was significantly decreased in tumor tissues. According to the mean expression of lncRNA CADM1-AS1, the patients were divided into a high expression group and a low expression group, and the expression of lncRNA CADM1-AS1 in gastric cancer was significantly correlated with tumor differentiation, N stage, M stage, and TNM stage. Moreover, the gastric cancer patients with higher expression of lncRNA CADM1-AS1 had a statistically better overall survival (OS) time and progression-free survival (PFS) time. In univariate analyses and multivariate analyses, the expression of lncRNA CADM1-AS1 was an independent prognosis index of patients with gastric cancer.
LncRNA CADM1-AS1 might be a new prognostic biomarker for gastric cancer.
探讨长链非编码RNA CADM1-AS1（lncRNA CADM1-AS1）在胃癌中的表达及其临床意义。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测lncRNA CADM1-AS1在胃癌组织及其癌旁正常组织中的表达差异。然后，采用卡方检验分析CADM1-AS1在胃癌中的表达与临床病理特征之间的相关性。此外，采用对数秩检验和Cox比例风险回归模型评估lncRNA CADM1-AS1预测胃癌患者术后预后的潜力。
lncRNA CADM1-AS1在肿瘤组织中的表达显著降低。根据lncRNA CADM1-AS1的平均表达水平，将患者分为高表达组和低表达组，lncRNA CADM1-AS1在胃癌中的表达与肿瘤分化、N分期、M分期和TNM分期显著相关。此外，lncRNA CADM1-AS1表达较高的胃癌患者的总生存期（OS）和无进展生存期（PFS）在统计学上明显更好。在单因素分析和多因素分析中，lncRNA CADM1-AS1的表达是胃癌患者的独立预后指标。
lncRNA CADM1-AS1可能是胃癌一种新的预后生物标志物。
