Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Virology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Chin Med Assoc. 2018 Mar;81(3):262-267. doi: 10.1016/j.jcma.2017.07.010. Epub 2017 Oct 13.
Herpes simplex virus type 2 (HSV-2) infectious disease is one of the most common viral sexually transmitted diseases. As regards, vaginal lactobacilli play an important role in protecting host against the urogenital pathogens; here we assessed the potential antiviral activity of Lactobacillus crispatus against HSV-2 infection in vitro.
Both Vero and HeLa cell lines were treated by L. crispatus before, during and after HSV-2 infection. The pre-incubation assay was also performed for the evaluating of virus adsorption by L. crispatus. Virus titer reduction in each stage was determined by a plaque reduction assay.
L. crispatus significantly decreased the infectivity of the HSV-2 in initial steps on both cell lines; however, no significant inhibition was ascertained during adsorption and multiplication process. The lactobacilli adhere on Vero cells two-fold stronger than HeLa and subsequently protect the Vero cells nearly 2.5 fold higher than HeLa cell against the virion. Co-incubation of HSV-2 with bacterial cells prior to virus inoculation significantly decreased the virus titer.
L. crispatus appears to inhibit the entry of the virus into cells by trapping HSV-2 particles. In addition, formation of L. crispatus microcolonies in the cell surface could block HSV-2 receptors and prevent viral entry to cells in initial infection steps.
单纯疱疹病毒 2 型（HSV-2）传染病是最常见的病毒性性传播疾病之一。就阴道乳杆菌而言，其在保护宿主免受泌尿生殖道病原体感染方面发挥着重要作用；在此，我们评估了卷曲乳杆菌对 HSV-2 感染的潜在抗病毒活性。
在 HSV-2 感染之前、期间和之后，用卷曲乳杆菌处理 Vero 和 HeLa 细胞系。还进行了预孵育实验来评估乳杆菌对病毒吸附的作用。通过空斑减少试验测定各阶段的病毒滴度减少。
卷曲乳杆菌显著降低了两种细胞系中 HSV-2 的初始感染性；然而，在吸附和增殖过程中未确定出显著的抑制作用。乳杆菌在 Vero 细胞上的黏附能力比 HeLa 细胞强两倍，随后对 Vero 细胞的保护作用比 HeLa 细胞高近 2.5 倍，抵御病毒粒子。在接种病毒之前将 HSV-2 与细菌细胞共孵育可显著降低病毒滴度。
卷曲乳杆菌似乎通过捕获 HSV-2 颗粒来抑制病毒进入细胞。此外，在细胞表面形成卷曲乳杆菌微菌落可能会阻止 HSV-2 受体，并在初始感染步骤中防止病毒进入细胞。
