miR-124中的一个基因变异降低了中国哈萨克族人群患食管鳞状细胞癌的易感性。

A Genetic Variant in miR-124 Decreased the Susceptibility to Esophageal Squamous Cell Carcinoma in a Chinese Kazakh Population.

作者信息

Wu Fei, Li Mei, You Weiyan, Ji Yu, Cui Xiaobin, Hu Jianming, Chen Yunzhao, Pang Lijuan, Li Shugang, Wei Yutao, Yang Lan, Li Feng

机构信息

1 Department of Pathology, Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine , Shihezi, China .

2 The First Affiliated Hospital, Shihezi University School of Medicine , Shihezi, China .

出版信息

Genet Test Mol Biomarkers. 2018 Jan;22(1):29-34. doi: 10.1089/gtmb.2017.0115. Epub 2017 Dec 11.

DOI:10.1089/gtmb.2017.0115

PMID:29227754

Abstract

AIMS

Esophageal squamous cell carcinoma (ESCC) is characterized by high prevalence and mortality worldwide, and it is very highly prevalent in China. ESCC is caused by various factors, including microRNAs (miRNAs) whose expression have been shown to play a major role in tumor generation. Single nucleotide polymorphisms (SNPs) in miRNAs could affect susceptibility to numerous cancers. This study aimed to evaluate the relationship between SNPs in miR-124 and ESCC risk in the Chinese Kazakh population.

METHODS

A total of 239 Chinese Kazakh patients with ESCC and 227 healthy Chinese Kazakh individuals were recruited in this study. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to analyze the miR-124 rs531564 genotype.

RESULTS

Allele G of the miR-124 rs531564 polymorphism significantly reduced the risk of ESCC in the Chinese Kazakh population [odds ratio (OR) = 0.711; 95% confidence interval (CI): 0.508-0.996; p = 0.047]. The dominant model indicated that the CG+GG genotypes were associated with significantly decreased ESCC risk compared to the CC genotype (adjusted OR = 0.586; 95% CI: 0.396-0.867; p = 0.007). Stratification analyses showed that compared with the CC genotype, the CG and CG+GG genotypes manifested reduced ESCC risks in the female group [CG vs. CC: OR = 0.472; 95% CI: 0.255-0.872; p = 0.016; (CG+GG) vs. CC: OR = 0.472; 95% CI: 0.255-0.872; p = 0.016] and the age group of <57 years old [CG vs. CC: OR = 0.456; 95% CI: 0.258-0.806; p = 0.006; (CG+GG) vs. CC: OR = 0.456; 95%CI: 0.258-0.806; p = 0.006]. The miR-124 rs531564 polymorphism showed no significant association with histological stage, lymph node metastasis, depth of invasion, or tumor/node/metastasis stage.

CONCLUSIONS

Our findings are the first to be reported that the miR-124 rs531564 polymorphism decreased ESCC risk in the Chinese Kazakh population.

摘要

目的

食管鳞状细胞癌（ESCC）在全球范围内具有高发病率和高死亡率，在中国的发病率也非常高。ESCC由多种因素引起，包括微小RNA（miRNA），其表达已被证明在肿瘤发生中起主要作用。miRNA中的单核苷酸多态性（SNP）可能影响多种癌症的易感性。本研究旨在评估中国哈萨克族人群中miR-124基因单核苷酸多态性与ESCC风险之间的关系。

方法

本研究共纳入239例中国哈萨克族ESCC患者和227例健康中国哈萨克族个体。采用基质辅助激光解吸/电离飞行时间质谱法分析miR-124 rs531564基因型。

结果

miR-124 rs531564多态性的G等位基因显著降低了中国哈萨克族人群患ESCC的风险[比值比（OR）=0.711；95%置信区间（CI）：0.508-0.996；p=0.047]。显性模型表明，与CC基因型相比，CG+GG基因型与ESCC风险显著降低相关（校正OR=0.586；95%CI：0.396-0.867；p=0.007）。分层分析显示，与CC基因型相比，CG和CG+GG基因型在女性组[CG与CC：OR=0.472；95%CI：0.255-0.872；p=0.016；（CG+GG）与CC：OR=0.472；95%CI：0.255-0.872；p=0.016]和年龄<57岁的年龄组[CG与CC：OR=0.456；95%CI：0.258-0.806；p=0.006；（CG+GG）与CC：OR=0.456；95%CI：0.258-0.806；p=0.006]中表现出ESCC风险降低。miR-124 rs531564多态性与组织学分期、淋巴结转移、浸润深度或肿瘤/淋巴结/转移分期无显著相关性。