DNA修饰酶和限制酶在谷氨酸钾缓冲液（KGB）中的活性。
Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer.
作者信息
Hanish J, McClelland M
机构信息
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
出版信息
Gene Anal Tech. 1988 Sep-Oct;5(5):105-7. doi: 10.1016/0735-0651(88)90005-2.
PMID:2903843
Abstract
A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.
摘要
一种主要由钾和谷氨酸组成的单一缓冲液被证明适用于几乎所有限制性内切酶、DNA甲基化酶以及许多其他DNA修饰酶的活性。这种缓冲液的成分与目前用于这些酶的缓冲液有很大不同。
Zotero 插件