Raaphorst G P, Azzam E I, Sargent M, Feeley M M
Medical Physics Department, Ottawa Regional Cancer Centre, Ontario, Canada.
Int J Radiat Biol. 1988 Dec;54(6):1031-40. doi: 10.1080/09553008814552411.
C3H-10T1/2 cells were grown to plateau phase and assessed for recovery from radiation damage under acidic and normal pH conditions. Repair of potentially lethal damage (PLD) was observed after X-ray doses of 5.0, 6.0 and 12.0 Gy and repair increased with dose. The repair at an acidic pH of 6.8 was greater than at the normal pH of 7.4. Repair of potentially transforming damage (PTD) was also observed when cells were held in plateau phase after irradiation. Repair of PTD was greater at the acidic pH than at the normal pH. When PTD recovery was plotted vs PLD recovery the results showed a good linear correlation with approximately twice as much PTDR. The experimental conditions allowing PLDR and PTDR indicate that the repair process may be error-free, since transformation was reduced in all experiments which showed recovery from PLD.
将C3H - 10T1/2细胞培养至平台期，并评估其在酸性和正常pH条件下从辐射损伤中恢复的情况。在5.0、6.0和12.0 Gy的X射线剂量后观察到潜在致死损伤（PLD）的修复，且修复随剂量增加。在酸性pH 6.8时的修复大于正常pH 7.4时的修复。当细胞在照射后处于平台期时，也观察到潜在转化损伤（PTD）的修复。在酸性pH下PTD的修复大于正常pH下的修复。当绘制PTD恢复与PLD恢复的关系图时，结果显示出良好的线性相关性，PTDR约为PLDR的两倍。允许PLDR和PTDR的实验条件表明修复过程可能是无差错的，因为在所有显示从PLD恢复的实验中转化都减少了。
