Raaphorst G P, Azzam E I
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Mar;49(3):383-93. doi: 10.1080/09553008514552611.
Mouse embryo (C3H 10T1/2) cells were exposed to anisotonic NaCl solutions or combined treatments of radiation and anisotonic solutions. Anisotonic treatment with 0.05 or 0.5 mol/l solutions did not cause transformation and only prolonged exposure at 1.5 mol/l caused significant increases in transformation. When cells were irradiated in the presence of 0.05 mol/l NaCl, increased transformation occurred than when cells were irradiated in medium. Thus, anisotonic treatment after irradiation resulted in fixation of potentially lethal and transformed radiation damage. Fixation of potentially transformed damage was greater for cells irradiated in the presence of 0.05 mol/l NaCl than for cells irradiated in medium. When the NaCl treatment after irradiation was delayed by incubation at 37 degrees C, recovery of potentially lethal and potentially transformed damage was observed.
将小鼠胚胎（C3H 10T1/2）细胞暴露于非等渗NaCl溶液中，或进行辐射与非等渗溶液的联合处理。用0.05或0.5 mol/l溶液进行非等渗处理不会导致细胞转化，只有在1.5 mol/l溶液中长时间暴露才会使转化率显著增加。当细胞在0.05 mol/l NaCl存在下受到辐射时，其转化率比在培养基中辐射时有所增加。因此，辐射后进行非等渗处理会导致潜在致死性和转化性辐射损伤的固定。对于在0.05 mol/l NaCl存在下受到辐射的细胞，潜在转化损伤的固定程度大于在培养基中辐射的细胞。当辐射后NaCl处理通过在37℃孵育而延迟时，可观察到潜在致死性和潜在转化损伤的恢复。
