一种用于短柄草的高效且可重复的镰刀菌属接种方法。

A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon.

作者信息

Rana Anuj, Karunakaran Aneesh, Fitzgerald Timothy L, Sabburg Rosalie, Aitken Elizabeth A B, Henry Robert J, Powell Jonathan J, Kazan Kemal

机构信息

Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, 306 Carmody Road, Brisbane, QLD, 4067, Australia.

Department of Genetics, University of Delhi, South Campus, New Delhi, 110021, India.

出版信息

Methods Mol Biol. 2018;1667:43-55. doi: 10.1007/978-1-4939-7278-4_4.

DOI:10.1007/978-1-4939-7278-4_4

PMID:29039002

Abstract

Fusarium spp. are devastating fungal pathogens which cause significant losses in many cereal crops like wheat, maize, and barley. Genetic improvement of disease resistance requires an improved understanding of defense-associated processes operating in the host in response to an attack by Fusarium spp. Brachypodium distachyon is emerging as a model where host-cereal-infecting pathogen interactions can be studied conveniently. However, this requires developing an efficient infection assay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible Fusarium infection assay for Brachypodium. We believe this method will help further develop Brachypodium as a model for genetic improvement of disease resistance in cereals against Fusarium pathogens.

摘要

镰刀菌属是具有破坏性的真菌病原体，会在许多谷类作物（如小麦、玉米和大麦）中造成重大损失。抗病性的遗传改良需要更好地理解宿主中与防御相关的过程，这些过程是宿主应对镰刀菌属攻击时所产生的。短柄草正在成为一种模式植物，通过它可以方便地研究宿主-谷类感染病原体之间的相互作用。然而，这需要开发一种高效的感染检测方法，以便于快速筛选种质（如突变系）。在此，我们为短柄草提供了一种高效且可重复的镰刀菌感染检测方法。我们相信这种方法将有助于进一步把短柄草发展成为一种用于谷类作物抗镰刀菌病原体遗传改良的模式植物。