Jimenez-Quiros Catherine, Okechukwu Emeka C, Hong Yiguo, Baysal Ömür, Tör Mahmut
Department of Biology, School of Science and the Environment, University of Worcester, Worcester WR2 6AJ, UK.
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Plants (Basel). 2022 Jul 31;11(15):1999. doi: 10.3390/plants11151999.
() causes Fusarium head blight (FHB) disease in wheat and barley. This pathogen produces mycotoxins including deoxynivalenol (DON), the T-2 and fumorisin B1. Translocation of the mycotoxins in grains causes important losses in yields and contributes to serious health problems in humans and livestock. We tested the strains, two commercial, QST713 (Serenade) and FZB24 (TAEGRO) and one non-commercial strain EU07 as microbial biological control agents against the strain K1-4 both in vitro and in planta. The EU07 strain showed better performance in suppressing the growth of -K1-4. Cell-free bacterial cultures displayed significant antagonistic activity on -K1-4. Remarkably, heat and proteinase K treatment of bacterial broths did not reduce the antagonistic activity of cultures. DON assays showed that strain was not affected by the presence of DON in the media. Leaf and head infection assays using (Bd-21) indicated that EU07 inhibits K1-4 growth in vivo and promotes plant growth. Overall, the EU07 strain performed better, indicating that it could be explored for the molecular investigations and protection of cereal crops against FHB disease.
（）在小麦和大麦中引发镰刀菌穗腐病（FHB）。这种病原体产生包括脱氧雪腐镰刀菌烯醇（DON）、T-2毒素和伏马菌素B1在内的霉菌毒素。霉菌毒素在谷物中的转运导致产量的重大损失，并对人类和牲畜的健康造成严重问题。我们测试了两种商业菌株QST713（小盾壳霉）和FZB24（TAEGRO）以及一种非商业菌株EU07作为微生物生物防治剂，在体外和植物体内对抗菌株K1-4。EU07菌株在抑制K1-4的生长方面表现出更好的性能。无细胞细菌培养物对K1-4显示出显著的拮抗活性。值得注意的是，对细菌培养液进行加热和蛋白酶K处理并没有降低培养物的拮抗活性。DON检测表明，该菌株不受培养基中DON存在的影响。使用（Bd-21）进行的叶片和穗部感染试验表明，EU07在体内抑制K1-4的生长并促进植物生长。总体而言，EU07菌株表现更好，表明它可用于分子研究以及保护谷类作物免受FHB病害。
