Ricker Erica B, Nuxoll Eric
a Department of Chemical and Biochemical Engineering , University of Iowa , Iowa City , IA , USA.
Biofouling. 2017 Nov;33(10):855-866. doi: 10.1080/08927014.2017.1381688. Epub 2017 Oct 17.
Upon formation of a biofilm, bacteria undergo several changes that prevent eradication with antimicrobials alone. Due to this resistance, the standard of care for infected medical implants is explantation of the infected implant and surrounding tissue, followed by eventual reimplantation of a replacement device. Recent studies have demonstrated the efficacy of heat shock for biofilm eradication. To minimize the heat required for in situ biofilm eradication, this study investigated the hypothesis that antibiotics, while ineffective by themselves, may substantially increase heat shock efficacy. The combined effect of heat and antibiotics on Pseudomonas aeruginosa biofilms was quantified via heat shock in combination with ciprofloxacin, tobramycin, or erythromycin at multiple concentrations. Combined treatments had synergistic effects for all antibiotics for heat shock conditions of 60°C for 5 min to 70°C for 1 min, indicating an alternative to surgical explantation.
在生物膜形成后，细菌会发生多种变化，从而使得仅使用抗菌药物无法将其根除。由于这种耐药性，对于感染的医用植入物，标准治疗方法是将感染的植入物及周围组织取出，随后最终重新植入替代装置。最近的研究已证明热休克对生物膜根除的有效性。为了将原位根除生物膜所需的热量降至最低，本研究调查了以下假设：抗生素自身虽无效，但可能会大幅提高热休克的效果。通过热休克结合多种浓度的环丙沙星、妥布霉素或红霉素，对热和抗生素对铜绿假单胞菌生物膜的联合作用进行了量化。对于60°C持续5分钟至70°C持续1分钟的热休克条件，联合治疗对所有抗生素均具有协同作用，这表明了一种替代手术取出的方法。
