Zhang Liwen, Zhao Mingxian, Aikeremu Feiruoran, Huang Huoshui, You Minsheng, Zhao Qian
State Key Laboratory for Ecological Pest Control of Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China.
Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, China.
Front Physiol. 2023 Jan 4;13:1068543. doi: 10.3389/fphys.2022.1068543. eCollection 2022.
Chemosensory proteins (CSPs) can bind and transport odorant molecules, which are believed to be involved in insect chemoreception. Here, we investigated three CSPs in perception of volatiles in . Expression profiles showed that although , and were ubiquitously expressed in heads, legs, thoraxes and abdomen, they were all highly expressed in the antennae of . Further, fluorescence competitive binding assays revealed that and 6-1 had binding affinities for three plant volatiles, suggesting their possible involvement in the chemosensory process. Among them, showed relatively high binding affinities for benzaldehyde. Behavioral assays revealed that the adults of showed a significant preference for two compounds including benzaldehyde. The predicted three-dimensional (3D) structures of these 3 CSP have the typical six α-helices, which form the hydrophobic ligand-binding pocket. We therefore suggest that might be involved in the chemoreception of the host-related volatiles for . Our data may provide a chance of finding a suitable antagonist of alternative control strategies which block the perception of chemosensory signals in pest, preventing the food- orientation behaviors.
化学感受蛋白（CSPs）能够结合并转运气味分子，据信其参与昆虫的化学感受过程。在此，我们研究了三种CSPs在[昆虫名称]对挥发性物质感知中的作用。表达谱显示，尽管[CSP1名称]、[CSP2名称]和[CSP3名称]在头部、腿部、胸部和腹部均有广泛表达，但它们在[昆虫名称]的触角中均高度表达。此外，荧光竞争结合试验表明，[CSP2名称]和6-1对三种植物挥发性物质具有结合亲和力，表明它们可能参与化学感受过程。其中，[CSP2名称]对苯甲醛表现出相对较高的结合亲和力。行为试验表明，[昆虫名称]成虫对包括苯甲醛在内的两种化合物表现出明显偏好。这三种CSP的预测三维（3D）结构具有典型的六个α-螺旋，形成疏水配体结合口袋。因此，我们认为[CSP2名称]可能参与[昆虫名称]对宿主相关挥发性物质的化学感受。我们的数据可能为寻找合适的拮抗剂提供机会，以阻断害虫化学感受信号的感知，从而阻止其食物定向行为，为替代控制策略提供依据。
