CsrA可使AcrAB多药耐药转运蛋白的表达最大化。

CsrA maximizes expression of the AcrAB multidrug resistance transporter.

作者信息

Ricci Vito, Attah Victoria, Overton Tim, Grainger David C, Piddock Laura J V

机构信息

Antimicrobials Research Group, School of Immunity and Infection, College of Medical and Dental Sciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham B15 2TT, UK.

Bioengineering, School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.

出版信息

Nucleic Acids Res. 2017 Dec 15;45(22):12798-12807. doi: 10.1093/nar/gkx929.

Carbon Storage Regulator A (CsrA) is an RNA binding protein that acts as a global regulator of diverse genes. Using a combination of genetics and biochemistry we show that CsrA binds directly to the 5' end of the transcript encoding AcrAB. Deletion of csrA or mutagenesis of the CsrA binding sites reduced production of both AcrA and AcrB. Nucleotide substitutions at the 5' UTR of acrA mRNA that could potentially weaken the inhibitory RNA secondary structure, allow for more efficient translation of the AcrAB proteins. Given the role of AcrAB-TolC in multi-drug efflux we suggest that CsrA is a potential drug target.