鼠李糖调节蛋白 RamA 在鼠伤寒沙门氏菌中的调节：RamR 超级阻遏物的分离。
Regulation of RamA by RamR in Salmonella enterica serovar Typhimurium: isolation of a RamR superrepressor.
机构信息
Antimicrobial Agents Research Group, School of Immunity and Infection and Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom.
出版信息
Antimicrob Agents Chemother. 2012 Nov;56(11):6037-40. doi: 10.1128/AAC.01320-12. Epub 2012 Sep 4.
Abstract
RamA is a transcription factor involved in regulating multidrug resistance in Salmonella enterica serovar Typhimurium SL1344. Green fluorescent protein (GFP) reporter fusions were exploited to investigate the regulation of RamA expression by RamR. We show that RamR represses the ramA promoter by binding to a palindromic sequence and describe a superrepressor RamR mutant that binds to the ramA promoter sequence more efficiently, thus exhibiting a ramA inactivated phenotype.
摘要
RamA 是一种转录因子，参与调节鼠伤寒沙门氏菌血清型 Typhimurium SL1344 的多药耐药性。绿色荧光蛋白 (GFP) 报告基因融合被用来研究 RamR 对 RamA 表达的调控。我们表明，RamR 通过结合一个回文序列来抑制 ramA 启动子，并描述了一个超级抑制 RamR 突变体，它能更有效地结合 ramA 启动子序列，从而表现出 ramA 失活表型。
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