Toxicology and Forensic Medicine Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
Biochemistry and Chemistry of Nutrition Department, Faculty Of Veterinary Medicine, Cairo University, Egypt.
Biomed Pharmacother. 2017 Dec;96:710-715. doi: 10.1016/j.biopha.2017.10.028. Epub 2017 Nov 6.
Introduction to the herbicide Atrazine (ATR) can bring about immunotoxicity, aside from other unfavorable results for the creature and human wellbeing. We went for clarifying the genotoxic mechanisms required in humoral immunotoxicity of Gesaprim (ATR) and their constriction by Akropwer. Forty rabbits (1.5kg±20%) were utilized and appointed into 4 equal groups. group 1: control; group 2: Received Atrazine at 1/10 LD via food; group 3: Received Akropwer at 1ml/1l/day by means of drinking water; group 4: Received both Atrazine and Akropwer associatively by the same said dosage and course. Atrazine and Akropower exposure were accomplished for 60days. The genotoxic mechanisms of Atrazine- induced humoral immunotoxicity were explained by increased serum total protein and albumin levels, decreased RHDV antibody titer only after four weeks of vaccination and increased level of spleen Fas and Caspase-III genes expression in Atrazine-exposed rabbits. Marked splenocytes apoptosis were detected in the immunohistochemical examination by caspase-III technique and TUNEL assay. Akropower attenuated ATR-induced apoptosis through down-regulation of Fas and Caspase-III genes expression and suppression of their signaling pathway. In conclusion, induction of apoptosis by overexpression of Fas and Caspase-III genes gives a new insight into the mechanism of ATR immunotoxicity. The protective part of Akropower, on the other hand, was characterized by attenuation of Fas and Caspase-III genes mediated apoptosis.
除草剂莠去津（ATR）除了对生物和人类健康造成其他不利影响外，还会导致免疫毒性。我们旨在阐明 Gesaprim（ATR）体液免疫毒性的遗传毒性机制及其被 Akropwer 抑制的机制。我们使用了 40 只兔子（1.5kg±20%），并将它们平均分为 4 组。组 1：对照组；组 2：通过食物摄入 1/10LD 的莠去津；组 3：通过饮用水摄入 1ml/1l/天的 Akropwer；组 4：通过相同剂量和疗程联合摄入莠去津和 Akropwer。莠去津和 Akropower 的暴露时间为 60 天。通过增加血清总蛋白和白蛋白水平、在接种后仅 4 周就降低 RHDV 抗体滴度以及增加暴露于莠去津的兔子脾脏 Fas 和 Caspase-III 基因表达水平，解释了莠去津诱导的体液免疫毒性的遗传毒性机制。通过 caspase-3 技术和 TUNEL 检测的免疫组化检查中检测到明显的脾细胞凋亡。Akropower 通过下调 Fas 和 Caspase-III 基因表达及其信号通路抑制来减轻 ATR 诱导的凋亡。总之，Fas 和 Caspase-III 基因的过度表达诱导凋亡为 ATR 免疫毒性的机制提供了新的见解。另一方面，Akropower 的保护作用表现为 Fas 和 Caspase-III 基因介导的凋亡的衰减。
