College of Resource and Environment, Jilin Agricultural University, Changchun, China.
Department of Computer Science, Iowa State University, Ames, IA, USA.
Biomed Res Int. 2019 Jul 28;2019:4756579. doi: 10.1155/2019/4756579. eCollection 2019.
Bacterial strain FH-1 with high efficiency of degrading Atrazine is separated by means of enrichment culture from the soil applied with Atrazine for many years. FH-1, recognized as based on phylogenetic analysis of 16S rDNA sequences, can grow with Atrazine which is the sole nitrogen source. In fluid inorganic salt medium, the optimal degradation temperature, pH value, and initial concentration of Atrazine are 25°C, 9.0, and 50 mg L, respectively, and the degradation rate of Atrazine by strain FH-1 reached 81.5% in 11 d of culture. The degrading process conforms to the kinetics equation of pesticide degradation. Among the metal ions tested, Zn (0.2 mM) has the most significant effect of facilitation on the degradation of Atrazine. In the fluid medium with Zn, the degradation rate of Atrazine is increased to 72.5%, while the Cu (0.2 mM) inhibits the degradation of Atrazine. The degradation products of Atrazine by strain FH-1 were identified as HEIT (2-hydroxyl-4-ethylamino-6-isopropylamino-1,3,5-triazine), MEET (2-hydroxyl-4,6-bis(ethylamino)-1,3,5-triazine), and AEEO (4,6-bis(ethylamino)-1,3,5-triazin-2(1H)-one) by HPLC-MS/MS. Three genes (, , and ) encoding for Atrazine degrading enzymes were identified by PCR and sequencing in strain FH-1. This study provides additional theoretical support for the application of strain FH-1 in bioremediation of fields polluted by Atrazine.
一株高效降解莠去津的细菌 FH-1 是通过从多年施加莠去津的土壤中进行富集培养分离得到的。FH-1 基于 16S rDNA 序列的系统发育分析被鉴定为。它可以利用莠去津作为唯一的氮源进行生长。在液体无机盐培养基中，其最适降解温度、pH 值和莠去津初始浓度分别为 25°C、9.0 和 50mg/L，FH-1 菌株在 11 天的培养中对莠去津的降解率达到 81.5%。该降解过程符合农药降解动力学方程。在所测试的金属离子中，Zn（0.2mM）对莠去津的降解具有最显著的促进作用。在含 Zn 的液体培养基中，莠去津的降解率提高到 72.5%，而 Cu（0.2mM）则抑制莠去津的降解。FH-1 菌株对莠去津的降解产物通过 HPLC-MS/MS 鉴定为 HEIT（2-羟基-4-乙基氨基-6-异丙基氨基-1,3,5-三嗪）、MEET（2-羟基-4,6-双（乙基氨基）-1,3,5-三嗪）和 AEEO（4,6-双（乙基氨基）-1,3,5-三嗪-2(1H)-酮）。通过 PCR 和测序，在 FH-1 菌株中鉴定出编码莠去津降解酶的三个基因（、和）。该研究为 FH-1 菌株在莠去津污染农田的生物修复中的应用提供了额外的理论支持。
