Rebordão M R, Alexandre-Pires G, Carreira M, Adriano L, Carneiro C, Nunes T, Mateus L, Ferreira-Dias G
CIISA, Faculty of Veterinary Medicine, University of Lisbon, Av. Universidade Técnica, 1300-477 Lisbon, Portugal; Clínica Veterinária das Nogueiras, R. Estádio 6, 3030-318 Coimbra, Portugal; Coimbra College of Agriculture, Bencanta, 3045-601 Coimbra, Portugal.
CIISA, Faculty of Veterinary Medicine, University of Lisbon, Av. Universidade Técnica, 1300-477 Lisbon, Portugal.
Vet Immunol Immunopathol. 2017 Oct;192:8-12. doi: 10.1016/j.vetimm.2017.09.001. Epub 2017 Sep 5.
Neutrophils are capable of releasing their DNA in response to infectious agents to form neutrophil extracellular traps (NETs) to destroy pathogens. Even though pyometra in queens and bitches is a common disease, its pathogenesis is not fully understood. The aim of this study was to assess the presence of NETs in the endometrium of queens and bitches suffering from pyometra. Pyometra and normal uteri were obtained after ovariohysterectomy from adult queens and bitches in diestrus. Uterine contents were evaluated for bacterial isolation and identification and for NETs presence. Escherichia coli were isolated in 5/5 queens and 4/5 bitches, and Streptococcus spp in one bitch. Sterile glass coverslips were placed on the endometrium surface to obtain material for NETs that were evaluated by immunocytochemistry (histone, neutrophil elastase or myeloperoxidase), fluorescence microscopy or scanning electron microscopy. NETs in endometrium content were positively stained by DNA histone DAPI, myeloperoxidase and by neutrophil elastase. NETs were spread in all observed queen and bitch endometria of pyometra cases. Ultrastructure images of NETs depicted clusters of globular material with fine filaments deposited on or around thick filaments and trapped bacteria. To the best of our knowledge these are the first findings confirming NETs endometrial presence in queen and bitch pyometra. Nevertheless, the precise role of NETs in pyometra in the bitch and queen, either to contribute to the defeat of infection or to its persistence remains to be unraveled.
中性粒细胞能够响应感染因子释放其DNA，形成中性粒细胞胞外陷阱（NETs）以破坏病原体。尽管母猫和母犬的子宫蓄脓是一种常见疾病，但其发病机制尚未完全明确。本研究的目的是评估患有子宫蓄脓的母猫和母犬子宫内膜中NETs的存在情况。在发情后期对成年母猫和母犬进行卵巢子宫切除术后获取子宫蓄脓和正常子宫样本。对子宫内容物进行细菌分离鉴定以及NETs存在情况的评估。在5只母猫中的5只以及5只母犬中的4只分离出大肠杆菌，在1只母犬中分离出链球菌属。将无菌玻璃盖玻片放置在子宫内膜表面以获取用于NETs检测的材料，通过免疫细胞化学（组蛋白、中性粒细胞弹性蛋白酶或髓过氧化物酶）、荧光显微镜或扫描电子显微镜进行评估。子宫内膜内容物中的NETs被DNA组蛋白DAPI、髓过氧化物酶和中性粒细胞弹性蛋白酶阳性染色。NETs分布于所有观察到的子宫蓄脓病例的母猫和母犬子宫内膜中。NETs的超微结构图像显示球状物质簇，细纤维沉积在粗纤维上或周围，并捕获了细菌。据我们所知，这些是首次证实母猫和母犬子宫蓄脓中NETs存在于子宫内膜的研究结果。然而，NETs在母犬和母猫子宫蓄脓中的确切作用，无论是有助于战胜感染还是导致感染持续，仍有待阐明。
