Nakamura Y, Carlson M, Krapcho K, Kanamori M, White R
Howard Hughes Medical Institute, University of Utah Health Sciences Center, Salt Lake City 84132.
Am J Hum Genet. 1988 Dec;43(6):854-9.
Elsewhere we have reported an efficient method for isolating VNTR (Variable Number of Tandem Repeats) markers. Several of the VNTR markers isolated in those experiments were sequenced, and a DNA sequence of 9 bp (GNNGTGGG) emerged as an apparent consensus sequence for VNTR markers. To confirm this result and to develop more VNTR markers, we synthesized nine different 18-base-long oligonucleotides whose sequences each included GNNGTGGG. When 102 cosmid clones selected by these oligonucleotides were tested for polymorphism, 34 (33%) of them showed multiallelic VNTR polymorphisms (average heterozygosity 68%). This procedure represents a new and efficient approach for isolating additional VNTR markers and supports the idea that the GNNGTGGG sequence may play an important role in the generation of the multiallelic systems within the human genome.
我们在其他地方报道了一种分离VNTR（可变串联重复序列）标记的有效方法。在那些实验中分离出的几个VNTR标记进行了测序，一个9bp（GNNGTGGG）的DNA序列成为VNTR标记的明显共有序列。为了证实这一结果并开发更多的VNTR标记，我们合成了9种不同的18个碱基长的寡核苷酸，其序列均包含GNNGTGGG。当测试由这些寡核苷酸选择的102个黏粒克隆的多态性时，其中34个（33%）显示出多等位基因VNTR多态性（平均杂合度68%）。该方法代表了一种分离更多VNTR标记的新的有效方法，并支持GNNGTGGG序列可能在人类基因组内多等位基因系统的产生中起重要作用这一观点。
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