检测菜豆（Lens culinaris Medik.）种质资源中的 DNA 多态性和遗传多样性：ISSR 和 DAMD 标记的比较。
Detecting DNA polymorphism and genetic diversity in Lentil (Lens culinaris Medik.) germplasm: comparison of ISSR and DAMD marker.
机构信息
Department of Agronomy & Plant Breeding, College of Agriculture & Natural Resources, Sanandaj Branch, Islamic Azad University, P.O. Box 618, Sanandaj, Iran.
出版信息
Physiol Mol Biol Plants. 2014 Oct;20(4):495-500. doi: 10.1007/s12298-014-0253-3. Epub 2014 Aug 8.
Genetic diversity and interrelationships among 31 lentil genotypes were evaluated using 10 Inter-Simple Sequence Repeat (ISSR) and 10 directed amplification of minisatellite DNA region (DAMD) primers. A total of 43 and 48 polymorphic bands were amplified by ISSR and DAMD markers, respectively. Average polymorphism information content (PIC) for ISSR and DAMD markers were 0.37 and 0.41, respectively. All 31 lentil genotypes could be distinguished by ISSR markers into three groups and by DAMD markers into two groups. Various molecular markers show a different efficiency for evaluating DNA polymorphism in lentil and indicate that the patterns of variation are clearly influenced by the genetic marker used. Comparatively, the genetic diversity of examined lentil genotypes by two different marker techniques (ISSR and DAMD) was high and indicated that ISSR and DAMD are effective and promising marker systems for fingerprinting in lentil and give useful information on its genetic relationships.
利用 10 对 ISSR 和 10 对 DAMD 引物，评估了 31 个兵豆基因型的遗传多样性和相互关系。ISSR 和 DAMD 标记分别扩增了 43 和 48 个多态性条带。ISSR 和 DAMD 标记的平均多态信息含量（PIC）分别为 0.37 和 0.41。ISSR 标记可将 31 个兵豆基因型分为三组，DAMD 标记可将其分为两组。各种分子标记在评估兵豆 DNA 多态性方面表现出不同的效率，表明变异模式明显受遗传标记的影响。相比之下，两种不同标记技术（ISSR 和 DAMD）检测到的兵豆基因型的遗传多样性较高，表明 ISSR 和 DAMD 是兵豆指纹图谱的有效且有前途的标记系统，并提供了其遗传关系的有用信息。
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