Tiwari Vandana, Meena Baleshwar, Nair Narayanan K, Rana Tikam S
Molecular Systematics Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh 226 001 India.
Physiol Mol Biol Plants. 2020 May;26(5):975-984. doi: 10.1007/s12298-020-00797-z. Epub 2020 Apr 18.
is an important medicinal plant species of Indian Himalayan Region (IHR). Genetic variability and population genetic structure of sampled from IHR was studied using two single primer amplification reaction (SPAR) methods (DAMD: Directed Amplification of Minisatellite region DNA; ISSR: Inter Simple Sequence Repeats). To provide a reasonable scientific basis for management and conservation of populations in IHR, genetic diversity analysis of 11 populations with 24 SPAR markers (15 ISSR and 9 DAMD) revealed significantly high level of (90.03%) polymorphism at species level. However, genetic variability was low at population level and KUL and BWS populations showed maximum while SHM population revealed least genetic diversity among the 11 populations. Analysis of molecular variance revealed highest percentage of variation (73%) within populations, followed by 17% among populations and least (10%) among the Himalayan regions. Clustering pattern obtained from UPGMA dendrogram was supported by STRUCTURE and principal coordinate analysis, segregating all the 11 natural populations of into two genetic clusters: Eastern and Western Himalayan populations. The clustering patterns of all the three statistical methods indicated that populations of have structured in response to the local micro-climates of the habitats in IHR, and therefore, it can be concluded that genetic variability is in congruence with the geographical diversity.
是印度喜马拉雅地区（IHR）一种重要的药用植物物种。使用两种单引物扩增反应（SPAR）方法（DAMD：微卫星区域DNA的定向扩增；ISSR：简单序列重复区间）对从IHR采集的样本进行了遗传变异性和群体遗传结构研究。为IHR地区该植物种群的管理和保护提供合理的科学依据，利用24个SPAR标记（15个ISSR和9个DAMD）对11个种群进行遗传多样性分析，结果显示在物种水平上多态性水平显著较高（90.03%）。然而，在种群水平上遗传变异性较低，在11个种群中，KUL和BWS种群的遗传多样性最高，而SHM种群的遗传多样性最低。分子方差分析显示，种群内变异百分比最高（73%），其次是种群间变异（17%）以及喜马拉雅地区间变异最小（10%）。UPGMA聚类图得到的聚类模式得到了STRUCTURE分析和主坐标分析的支持，将该植物的所有11个自然种群分为两个遗传簇：东喜马拉雅种群和西喜马拉雅种群。这三种统计方法得到的聚类模式均表明该植物种群是根据IHR地区栖息地局部微气候形成结构的，因此，可以得出结论：遗传变异性与地理多样性一致。
