Azemikhah Mitra, Ashtiani Hamidreza Ahmadi, Aghaei Mahmoud, Rastegar Hosein
Department of Biology, science and research branch, Islamic Azad University, Tehran, I.R. Iran.
Islamic Azad University, pharmaceutical sciences branch, Tehran, I.R. Iran.
Res Pharm Sci. 2015 Jul-Aug;10(4):356-63.
Discoidin domain receptor (DDR) is a new member of the receptor tyrosine kinase family. There are two isoforms of discoidin domain receptor (DDR), DDR1 and DDR2. These receptors play a major role in the adhesion, motility and cell proliferation. Due to the important role of DDR2 in the development of tumor extension, this receptor is pivotal in the field of carcinogenesis. The aim of this study was to investigate the mRNA and protein expression of DDR2, in the malignant, benign prostatic hyperplasia (BPH) and normal tissues of patients with prostate cancer. In this study the gene and protein expression of DDR2 in adjacent normal (n=40), BPH (n=40), and malignant (n=40) prostate tissue were measured using real-time PCR and Western blotting. Then, the correlation of DDR2 gene and protein expression with prognostic factors such as age, tumor grade, tumor stage, lymph node involvement, and serum prostate-specific antigen (PSA) concentration were evaluated. The relative mRNA and protein expression level of DDR2 in malignant and benign prostate tissue was significantly higher than those of adjacent normal tissues (P<0.01). This expression was found to increase approximately 3.5 and 2.1 fold for mRNA and protein levels, respectively. Spearman test indicated a significant correlation between DDR2 mRNA and protein expression with prognostic factors such as tumor grade, stage, lymph node involvement, and serum PSA concentration. However, significant correlation with age was not observed. These findings suggest that DDR2 is a cancer-related gene associated with the aggressive progression of prostate cancer patients.
盘状结构域受体（DDR）是受体酪氨酸激酶家族的新成员。盘状结构域受体（DDR）有两种亚型，DDR1和DDR2。这些受体在细胞黏附、迁移和增殖中起主要作用。由于DDR2在肿瘤侵袭发展中起重要作用，该受体在致癌作用领域至关重要。本研究旨在调查DDR2在前列腺癌患者的恶性、良性前列腺增生（BPH）和正常组织中的mRNA和蛋白表达。在本研究中，采用实时PCR和蛋白质印迹法检测DDR2在40例相邻正常前列腺组织、40例BPH组织和40例恶性前列腺组织中的基因和蛋白表达。然后，评估DDR2基因和蛋白表达与年龄、肿瘤分级、肿瘤分期、淋巴结受累及血清前列腺特异性抗原（PSA）浓度等预后因素的相关性。DDR2在恶性和良性前列腺组织中的相对mRNA和蛋白表达水平显著高于相邻正常组织（P<0.01）。mRNA和蛋白水平的表达分别增加约3.5倍和2.1倍。Spearman检验表明，DDR2 mRNA和蛋白表达与肿瘤分级、分期、淋巴结受累及血清PSA浓度等预后因素之间存在显著相关性。然而，未观察到与年龄的显著相关性。这些发现表明，DDR2是一个与前列腺癌患者侵袭性进展相关的癌相关基因。
