Liu Ziru, Yang Zhulin, Jiang Song, Zou Qiong, Yuan Yuan, Li Jinghe, Li Daiqiang, Liang Lufeng, Chen Meigui, Chen Senlin
Department of Minimal Invasive Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Research Laboratory of Hepatobiliary Diseases, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Mol Med Rep. 2016 Nov;14(5):4581-4592. doi: 10.3892/mmr.2016.5851. Epub 2016 Oct 13.
The clinicopathological and biological characteristics of squamous cell/adenosquamous carcinoma (SC/ASC) of the gallbladder remain to be fully elucidated, due to the fact that it is a rare gallbladder cancer subtype. In the current study, the expression of minichromosome maintenance complex component 2 (MCM2) and HIV‑1 tat interactive protein 2 (TIP30) was measured in 46 cases of SC/ASC and 80 adenocarcinomas (AC) using immunohistochemistry. Positive MCM2 and negative TIP30 expression were significantly associated with large tumor size, high TNM stage, invasion, lymph node metastasis and lack of surgical curability in SC/ASC and AC. Positive MCM2 and negative TIP30 expression were significantly associated with poor differentiation in AC, whereas only MCM2 was correlated with differentiation in SC/ASC. Univariate Kaplan‑Meier analysis demonstrated that positive MCM2 and negative TIP30 expression, the degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis and surgical curability were significantly associated with post‑operative survival in patients with SC/ASC and AC. Multivariate Cox regression analysis demonstrated that positive MCM2 and negative TIP30 expression, the degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis and lack of surgical curability were also independent predictors of poor prognosis in patients with SC/ASC and AC. These data suggest that positive MCM2 and negative TIP30 expression are closely correlated with the clinical, pathological and biological parameters, in addition to poor prognosis in patients with gallbladder cancer.
由于胆囊鳞状细胞/腺鳞癌（SC/ASC）是一种罕见的胆囊癌亚型，其临床病理和生物学特征仍有待充分阐明。在本研究中，采用免疫组织化学方法检测了46例SC/ASC和80例腺癌（AC）中微小染色体维持复合物组分2（MCM2）和HIV-1 tat相互作用蛋白2（TIP30）的表达。MCM2阳性和TIP30阴性表达与SC/ASC和AC的肿瘤体积大、TNM分期高、浸润、淋巴结转移及手术不可治愈性显著相关。MCM2阳性和TIP30阴性表达与AC的低分化显著相关，而在SC/ASC中仅MCM2与分化相关。单因素Kaplan-Meier分析表明，MCM2阳性和TIP30阴性表达、分化程度、肿瘤大小、TNM分期、浸润、淋巴结转移及手术可治愈性与SC/ASC和AC患者的术后生存显著相关。多因素Cox回归分析表明，MCM2阳性和TIP30阴性表达、分化程度、肿瘤大小、TNM分期、浸润、淋巴结转移及手术不可治愈性也是SC/ASC和AC患者预后不良的独立预测因素。这些数据表明，MCM2阳性和TIP30阴性表达除了与胆囊癌患者的预后不良密切相关外，还与临床、病理和生物学参数密切相关。
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