使用深度测序进行DNA复制谱分析。

DNA Replication Profiling Using Deep Sequencing.

作者信息

Saayman Xanita, Ramos-Pérez Cristina, Brown Grant W

机构信息

Department of Biochemistry and Donnelly Centre, University of Toronto, Toronto, ON, Canada, M5S 3E1.

出版信息

Methods Mol Biol. 2018;1672:195-207. doi: 10.1007/978-1-4939-7306-4_15.

DOI:10.1007/978-1-4939-7306-4_15

PMID:29043626

Abstract

Profiling of DNA replication during progression through S phase allows a quantitative snap-shot of replication origin usage and DNA replication fork progression. We present a method for using deep sequencing data to profile DNA replication in S. cerevisiae.

摘要

在S期进程中对DNA复制进行分析，能够定量获取复制起点使用情况和DNA复制叉进展的瞬时图像。我们提出了一种利用深度测序数据对酿酒酵母中的DNA复制进行分析的方法。