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四种植物提取物对阿昔洛韦耐药的1型单纯疱疹病毒的抗病毒筛选

Antiviral screening of four plant extracts against acyclovir resistant herpes simplex virus type-1.

作者信息

Parsania Masoud, Rezaee Mohammad-Bagher, Monavari Seyed Hamidreza, Jaimand Kamkar, Mousavi-Jazayeri Seyed Milad, Razazian Mehdi, Nadjarha Mohammad Hossein

机构信息

Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

出版信息

Pak J Pharm Sci. 2017 Jul;30(4(Suppl.)):1407-1411.

Abstract

Herpes simplex virus type 1 (HSV-1) causes serious infections particularly in immunocompromised patients. Methanolic extract of four plants were evaluated for their anti-viral effects against acyclovir resistant HSV-1 in HeLa cell line. The 50% cytotoxic concentration (CC50) as well as the effective minimal cytotoxic concentration of each plant extract were evaluated by MTT assay. Antiviral effects of the plant extracts on HSV-1 were examined at different concentrations of the extract. The effective minimal cytotoxic concentration was evaluated at different times of virus replication after infection. Virus titration was assessed by tissue culture infectious dose 50 (TCID50) method. Among the 4 plant extracts evaluated only Mentha pulegium L. extract was shown to exert the highest antiviral activity, with selectivity index (SI) 10.25. Direct treatment of HSV-1 with Mentha pulegium L. extract resulted in 1.7 log10 TCID50 reduction in virus titers after one hour. The highest reduction in HSV-1 infectivity was obtained 1 hour after the infection of the cells with virus resulting in 2.1 log10 TCID50 reduction as compared to the control. The antiviral effects of Mentha pulegium L. extract on HSV-1 after virus infection was more remarkable than the virucidal activity.

摘要

单纯疱疹病毒1型(HSV-1)尤其会在免疫功能低下的患者中引发严重感染。对四种植物的甲醇提取物针对HeLa细胞系中对阿昔洛韦耐药的HSV-1的抗病毒作用进行了评估。通过MTT法评估每种植物提取物的50%细胞毒性浓度(CC50)以及有效最小细胞毒性浓度。在提取物的不同浓度下检测植物提取物对HSV-1的抗病毒作用。在感染后病毒复制的不同时间评估有效最小细胞毒性浓度。通过组织培养感染剂量50(TCID50)法评估病毒滴度。在所评估的4种植物提取物中,仅唇萼薄荷提取物显示出最高的抗病毒活性,选择性指数(SI)为10.25。用唇萼薄荷提取物直接处理HSV-1,1小时后病毒滴度降低1.7 log10 TCID50。与对照相比,在用病毒感染细胞1小时后获得了HSV-1感染性的最大降低,导致2.1 log10 TCID50降低。病毒感染后唇萼薄荷提取物对HSV-1的抗病毒作用比杀病毒活性更显著。

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