Efficacy of traditional herb aqua extract of and its fractions against HSV-1 virus expression levels of genes (UL46 and US6).

BACKGROUND AND OBJECTIVES

Recently, the anti-herpetic activities of different plant species have been investigated. This study evaluated the effects of aqueous extract on the HSV-1 virus-infected Vero cell.

MATERIALS AND METHODS

The IC of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the MTT method, also the q-PCR was used to study viral gene expression reduction.

RESULTS

Results of the MTT test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of 's aqueous extracts (IC=45.5μg/ml). Time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (MOI=0.1), log 10, p <0.001). Furthermore, the plant's aqueous extract concentration almost avoids cell viability reduction. Through Q-PCR results; the reduction of viral proliferation revealed that the low expression of genes UL46 and US6 were significant in the presence of different treatments utilized in the experiment.

CONCLUSION

, has an anti-viral property and may be considered as a remedy for anti-HSV-1 agents.